A bifunctional metal organic framework of type Fe(III)-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose

• Published in: Mikrochimica acta (1966) 2019-05, Vol.186 (5), p.295-295, Article 295
• Main Authors: Zhao, Zhenghong, Pang, Junhong, Liu, Wenren, Lin, Tianran, Ye, Fanggui, Zhao, Shulin
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Benzidines - chemistry; Biomimetic Materials - metabolism; Biosensing Techniques - methods; Catalysis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Color; Colorimetry; Coloring Agents - chemistry; Glucose - analysis; Glucose Oxidase - metabolism; Hydrogen Peroxide; Iron - chemistry; Limit of Detection; Metal-Organic Frameworks - chemistry; Microengineering; Nanochemistry; Nanotechnology; Original Paper; Oxidation-Reduction; Peroxidases - metabolism; Tricarboxylic Acids - chemistry
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-019-3416-7

A metal organic framework (MOF) of type Fe(III)-BTC (where BTC is 1,3,5-benzenetricarboxylic acid) was utilized to construct an integrated system for cascade colorimetric determination of glucose. The MOF performs a dual function in acting (a) as a peroxidase (POx) mimic, and (b) as a solid support for immobilization of glucose oxidase (GOx). The MOF was prepared by a one-pot method. Glucose is consumed while H 2 O 2 is produced during the enzymatic oxidation by GOx. In the presence of H 2 O 2 , the POx mimic catalytically oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) to form a blue-green product. The absorbance of oxidized TMB (measured at 652 nm) increases linearly in the 5.0–100 μM glucose concentration range, and the detection limit is 2.4 μM. The GOx@Fe-BTC MOF was successfully applied to the determination of glucose in serum. Graphical abstract Schematic presentation of a bifunctional metal organic framework of type Fe-BTC for cascade (enzymatic and enzyme-mimicking) colorimetric determination of glucose. The Fe-BTC performs a dual function in acting as both a peroxidase mimic and support for immobilizing glucose oxidase. Using the integrated enzyme, a colorimetric method was successfully applied to one-step detection of glucose in human serum.