GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells

• Published in: Journal of physiology and biochemistry 2019-06, Vol.75 (2), p.209-215
• Main Authors: Villagrán, Marcelo, Muñoz, Mirna, Inostroza, Eveling, Venegas, Camila, Ruminot, Iván, Parra-Valencia, Esteban, Maldonado, Mafalda, del Pozo, Reginald, Rivas, Coralia I., Vera, Juan Carlos, Mardones, Lorena
• Format: Article
• Language: English
• Subjects: Alveoli; Animal Physiology; Animals; Biomedical and Life Sciences; Biomedicine; Breastfeeding & lactation; Carbohydrates; Cell proliferation; Cytokeratin; Energy requirements; Epithelial cells; Epithelial Cells - immunology; Epithelial Cells - metabolism; Female; Galactose; Gene expression; Glucose; Glucose - metabolism; Glucose Transport Proteins, Facilitative - genetics; Glucose Transport Proteins, Facilitative - metabolism; Glucose Transporter Type 1 - genetics; Glucose Transporter Type 1 - metabolism; Golgi Apparatus - metabolism; Golgi cells; Human Physiology; Immunohistochemistry; K protein; Keratin-18 - metabolism; Lactalbumin; Lactalbumin - metabolism; Lactation; Lactose; Lactose - biosynthesis; Lactose synthase; Lactose Synthase - metabolism; Localization; Mammary gland; Mammary glands; Mammary Glands, Animal - metabolism; Membrane proteins; Mice; Mice, Inbred BALB C; mRNA; Nutrients; Original Article; Peptides - metabolism; Pregnancy; Protein folding; Proteins; Retinoblastoma-Like Protein p130 - metabolism; RNA, Messenger - metabolism; Synthesis; Time Factors; Underpotential deposition; Weaning
• ISSN: 1138-7548; 1877-8755
• DOI: 10.1007/s13105-019-00679-3

The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation ( p