Loading…
GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells
The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose...
Saved in:
| Published in: | Journal of physiology and biochemistry 2019-06, Vol.75 (2), p.209-215 |
|---|---|
| Main Authors: | , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3 |
| container_end_page | 215 |
| container_issue | 2 |
| container_start_page | 209 |
| container_title | Journal of physiology and biochemistry |
| container_volume | 75 |
| creator | Villagrán, Marcelo Muñoz, Mirna Inostroza, Eveling Venegas, Camila Ruminot, Iván Parra-Valencia, Esteban Maldonado, Mafalda del Pozo, Reginald Rivas, Coralia I. Vera, Juan Carlos Mardones, Lorena |
| description | The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation (
p
|
| doi_str_mv | 10.1007/s13105-019-00679-3 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2215026377</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2215026377</sourcerecordid><originalsourceid>FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3</originalsourceid><addsrcrecordid>eNp9kD1PwzAURS0EoqXwBxiQJRaWgD_i2BlRBQWpEks7W47zUlLlCzsZ-u9xSAGJgclP8rnXzweha0ruKSHywVNOiYgITSNCEplG_ATNqZIyUlKI0zBTriIpYjVDF97vCYkZZeQczUKOkYTxOdqu1tsNxabJ8Tgp7Ieua12PK2P71gP2h6Z_B196XDZ41Va7ErcFrgdXNoBrU9fGHTB0ZYCq0lTYQlX5S3RWmMrD1fFcoO3z02b5Eq3fVq_Lx3VkuRR9FBsJwgoruc0JtbIoBAWRMsNjC4LFuWIZQCEhUaxIM0lMHqdpIpnJpCmE4Qt0N_V2rv0YwPe6Lv24gWmgHbxmjArCEi5lQG__oPt2cE3YLlCCJYoSlQSKTZR1rfcOCt25cvyipkSP0vUkXQfp-ku65iF0c6weshryn8i35QDwCfDhqtmB-337n9pPbFSLww</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2252681086</pqid></control><display><type>article</type><title>GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells</title><source>Springer Nature</source><creator>Villagrán, Marcelo ; Muñoz, Mirna ; Inostroza, Eveling ; Venegas, Camila ; Ruminot, Iván ; Parra-Valencia, Esteban ; Maldonado, Mafalda ; del Pozo, Reginald ; Rivas, Coralia I. ; Vera, Juan Carlos ; Mardones, Lorena</creator><creatorcontrib>Villagrán, Marcelo ; Muñoz, Mirna ; Inostroza, Eveling ; Venegas, Camila ; Ruminot, Iván ; Parra-Valencia, Esteban ; Maldonado, Mafalda ; del Pozo, Reginald ; Rivas, Coralia I. ; Vera, Juan Carlos ; Mardones, Lorena</creatorcontrib><description>The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation (
p
< 0.05) and 2-fold at protein level for GLUT1 and GLUT8 (
p
< 0.05 and 0.01, respectively). The temporal expression pattern was shared with cytokeratin 18 and Ki67 (
p
< 0.01). Endogenous GLUT8 partially co-localized with 58 K protein and α-lactalbumin in mammary tissue and with Golgi membrane–associated protein 130 in isolated epithelial cells. The spatial-temporal synchrony between expression of GLUT8/GLUT1 and alveolar cell proliferation, and its localization in
cis-
Golgi associated to lactose synthase complex, suggest that both transporters are involved in glucose uptake into this organelle, supporting lactose synthesis.</description><identifier>ISSN: 1138-7548</identifier><identifier>EISSN: 1877-8755</identifier><identifier>DOI: 10.1007/s13105-019-00679-3</identifier><identifier>PMID: 31020623</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alveoli ; Animal Physiology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Breastfeeding & lactation ; Carbohydrates ; Cell proliferation ; Cytokeratin ; Energy requirements ; Epithelial cells ; Epithelial Cells - immunology ; Epithelial Cells - metabolism ; Female ; Galactose ; Gene expression ; Glucose ; Glucose - metabolism ; Glucose Transport Proteins, Facilitative - genetics ; Glucose Transport Proteins, Facilitative - metabolism ; Glucose Transporter Type 1 - genetics ; Glucose Transporter Type 1 - metabolism ; Golgi Apparatus - metabolism ; Golgi cells ; Human Physiology ; Immunohistochemistry ; K protein ; Keratin-18 - metabolism ; Lactalbumin ; Lactalbumin - metabolism ; Lactation ; Lactose ; Lactose - biosynthesis ; Lactose synthase ; Lactose Synthase - metabolism ; Localization ; Mammary gland ; Mammary glands ; Mammary Glands, Animal - metabolism ; Membrane proteins ; Mice ; Mice, Inbred BALB C ; mRNA ; Nutrients ; Original Article ; Peptides - metabolism ; Pregnancy ; Protein folding ; Proteins ; Retinoblastoma-Like Protein p130 - metabolism ; RNA, Messenger - metabolism ; Synthesis ; Time Factors ; Underpotential deposition ; Weaning</subject><ispartof>Journal of physiology and biochemistry, 2019-06, Vol.75 (2), p.209-215</ispartof><rights>University of Navarra 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3</citedby><cites>FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3</cites><orcidid>0000-0001-8037-4296</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31020623$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villagrán, Marcelo</creatorcontrib><creatorcontrib>Muñoz, Mirna</creatorcontrib><creatorcontrib>Inostroza, Eveling</creatorcontrib><creatorcontrib>Venegas, Camila</creatorcontrib><creatorcontrib>Ruminot, Iván</creatorcontrib><creatorcontrib>Parra-Valencia, Esteban</creatorcontrib><creatorcontrib>Maldonado, Mafalda</creatorcontrib><creatorcontrib>del Pozo, Reginald</creatorcontrib><creatorcontrib>Rivas, Coralia I.</creatorcontrib><creatorcontrib>Vera, Juan Carlos</creatorcontrib><creatorcontrib>Mardones, Lorena</creatorcontrib><title>GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells</title><title>Journal of physiology and biochemistry</title><addtitle>J Physiol Biochem</addtitle><addtitle>J Physiol Biochem</addtitle><description>The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation (
p
< 0.05) and 2-fold at protein level for GLUT1 and GLUT8 (
p
< 0.05 and 0.01, respectively). The temporal expression pattern was shared with cytokeratin 18 and Ki67 (
p
< 0.01). Endogenous GLUT8 partially co-localized with 58 K protein and α-lactalbumin in mammary tissue and with Golgi membrane–associated protein 130 in isolated epithelial cells. The spatial-temporal synchrony between expression of GLUT8/GLUT1 and alveolar cell proliferation, and its localization in
cis-
Golgi associated to lactose synthase complex, suggest that both transporters are involved in glucose uptake into this organelle, supporting lactose synthesis.</description><subject>Alveoli</subject><subject>Animal Physiology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Breastfeeding & lactation</subject><subject>Carbohydrates</subject><subject>Cell proliferation</subject><subject>Cytokeratin</subject><subject>Energy requirements</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - immunology</subject><subject>Epithelial Cells - metabolism</subject><subject>Female</subject><subject>Galactose</subject><subject>Gene expression</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Glucose Transport Proteins, Facilitative - genetics</subject><subject>Glucose Transport Proteins, Facilitative - metabolism</subject><subject>Glucose Transporter Type 1 - genetics</subject><subject>Glucose Transporter Type 1 - metabolism</subject><subject>Golgi Apparatus - metabolism</subject><subject>Golgi cells</subject><subject>Human Physiology</subject><subject>Immunohistochemistry</subject><subject>K protein</subject><subject>Keratin-18 - metabolism</subject><subject>Lactalbumin</subject><subject>Lactalbumin - metabolism</subject><subject>Lactation</subject><subject>Lactose</subject><subject>Lactose - biosynthesis</subject><subject>Lactose synthase</subject><subject>Lactose Synthase - metabolism</subject><subject>Localization</subject><subject>Mammary gland</subject><subject>Mammary glands</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Membrane proteins</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>mRNA</subject><subject>Nutrients</subject><subject>Original Article</subject><subject>Peptides - metabolism</subject><subject>Pregnancy</subject><subject>Protein folding</subject><subject>Proteins</subject><subject>Retinoblastoma-Like Protein p130 - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Synthesis</subject><subject>Time Factors</subject><subject>Underpotential deposition</subject><subject>Weaning</subject><issn>1138-7548</issn><issn>1877-8755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kD1PwzAURS0EoqXwBxiQJRaWgD_i2BlRBQWpEks7W47zUlLlCzsZ-u9xSAGJgclP8rnXzweha0ruKSHywVNOiYgITSNCEplG_ATNqZIyUlKI0zBTriIpYjVDF97vCYkZZeQczUKOkYTxOdqu1tsNxabJ8Tgp7Ieua12PK2P71gP2h6Z_B196XDZ41Va7ErcFrgdXNoBrU9fGHTB0ZYCq0lTYQlX5S3RWmMrD1fFcoO3z02b5Eq3fVq_Lx3VkuRR9FBsJwgoruc0JtbIoBAWRMsNjC4LFuWIZQCEhUaxIM0lMHqdpIpnJpCmE4Qt0N_V2rv0YwPe6Lv24gWmgHbxmjArCEi5lQG__oPt2cE3YLlCCJYoSlQSKTZR1rfcOCt25cvyipkSP0vUkXQfp-ku65iF0c6weshryn8i35QDwCfDhqtmB-337n9pPbFSLww</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Villagrán, Marcelo</creator><creator>Muñoz, Mirna</creator><creator>Inostroza, Eveling</creator><creator>Venegas, Camila</creator><creator>Ruminot, Iván</creator><creator>Parra-Valencia, Esteban</creator><creator>Maldonado, Mafalda</creator><creator>del Pozo, Reginald</creator><creator>Rivas, Coralia I.</creator><creator>Vera, Juan Carlos</creator><creator>Mardones, Lorena</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8037-4296</orcidid></search><sort><creationdate>20190601</creationdate><title>GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells</title><author>Villagrán, Marcelo ; Muñoz, Mirna ; Inostroza, Eveling ; Venegas, Camila ; Ruminot, Iván ; Parra-Valencia, Esteban ; Maldonado, Mafalda ; del Pozo, Reginald ; Rivas, Coralia I. ; Vera, Juan Carlos ; Mardones, Lorena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Alveoli</topic><topic>Animal Physiology</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Breastfeeding & lactation</topic><topic>Carbohydrates</topic><topic>Cell proliferation</topic><topic>Cytokeratin</topic><topic>Energy requirements</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - immunology</topic><topic>Epithelial Cells - metabolism</topic><topic>Female</topic><topic>Galactose</topic><topic>Gene expression</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Glucose Transport Proteins, Facilitative - genetics</topic><topic>Glucose Transport Proteins, Facilitative - metabolism</topic><topic>Glucose Transporter Type 1 - genetics</topic><topic>Glucose Transporter Type 1 - metabolism</topic><topic>Golgi Apparatus - metabolism</topic><topic>Golgi cells</topic><topic>Human Physiology</topic><topic>Immunohistochemistry</topic><topic>K protein</topic><topic>Keratin-18 - metabolism</topic><topic>Lactalbumin</topic><topic>Lactalbumin - metabolism</topic><topic>Lactation</topic><topic>Lactose</topic><topic>Lactose - biosynthesis</topic><topic>Lactose synthase</topic><topic>Lactose Synthase - metabolism</topic><topic>Localization</topic><topic>Mammary gland</topic><topic>Mammary glands</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Membrane proteins</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>mRNA</topic><topic>Nutrients</topic><topic>Original Article</topic><topic>Peptides - metabolism</topic><topic>Pregnancy</topic><topic>Protein folding</topic><topic>Proteins</topic><topic>Retinoblastoma-Like Protein p130 - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Synthesis</topic><topic>Time Factors</topic><topic>Underpotential deposition</topic><topic>Weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Villagrán, Marcelo</creatorcontrib><creatorcontrib>Muñoz, Mirna</creatorcontrib><creatorcontrib>Inostroza, Eveling</creatorcontrib><creatorcontrib>Venegas, Camila</creatorcontrib><creatorcontrib>Ruminot, Iván</creatorcontrib><creatorcontrib>Parra-Valencia, Esteban</creatorcontrib><creatorcontrib>Maldonado, Mafalda</creatorcontrib><creatorcontrib>del Pozo, Reginald</creatorcontrib><creatorcontrib>Rivas, Coralia I.</creatorcontrib><creatorcontrib>Vera, Juan Carlos</creatorcontrib><creatorcontrib>Mardones, Lorena</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villagrán, Marcelo</au><au>Muñoz, Mirna</au><au>Inostroza, Eveling</au><au>Venegas, Camila</au><au>Ruminot, Iván</au><au>Parra-Valencia, Esteban</au><au>Maldonado, Mafalda</au><au>del Pozo, Reginald</au><au>Rivas, Coralia I.</au><au>Vera, Juan Carlos</au><au>Mardones, Lorena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells</atitle><jtitle>Journal of physiology and biochemistry</jtitle><stitle>J Physiol Biochem</stitle><addtitle>J Physiol Biochem</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>75</volume><issue>2</issue><spage>209</spage><epage>215</epage><pages>209-215</pages><issn>1138-7548</issn><eissn>1877-8755</eissn><abstract>The mammary gland increases energy requirements during pregnancy and lactation to support epithelial proliferation and milk nutrients synthesis. Lactose, the principal carbohydrate of the milk, is synthetized in the Golgi of mammary epithelial cells by lactose synthase from glucose and UPD galactose. We studied the temporal changes in the expression of GLUT1 and GLUT8 in mammary gland and their association with lactose synthesis and proliferation in BALB/c mice. Six groups were used: virgin, pregnant at 2 and 17 days, lactating at 2 and 10 days, and weaning at 2 days. Temporal expression of GLUT1 and GLUT8 transporters by qPCR, western blot and immunohistochemistry, and its association with lactalbumin, Ki67, and cytokeratin 18 within mammary tissue was studied, along with subcellular localization. GLUT1 and GLUT8 transporters increased their expression during mammary gland progression, reaching 20-fold increasing in GLUT1 mRNA at lactation (
p
< 0.05) and 2-fold at protein level for GLUT1 and GLUT8 (
p
< 0.05 and 0.01, respectively). The temporal expression pattern was shared with cytokeratin 18 and Ki67 (
p
< 0.01). Endogenous GLUT8 partially co-localized with 58 K protein and α-lactalbumin in mammary tissue and with Golgi membrane–associated protein 130 in isolated epithelial cells. The spatial-temporal synchrony between expression of GLUT8/GLUT1 and alveolar cell proliferation, and its localization in
cis-
Golgi associated to lactose synthase complex, suggest that both transporters are involved in glucose uptake into this organelle, supporting lactose synthesis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31020623</pmid><doi>10.1007/s13105-019-00679-3</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-8037-4296</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1138-7548 |
| ispartof | Journal of physiology and biochemistry, 2019-06, Vol.75 (2), p.209-215 |
| issn | 1138-7548 1877-8755 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2215026377 |
| source | Springer Nature |
| subjects | Alveoli Animal Physiology Animals Biomedical and Life Sciences Biomedicine Breastfeeding & lactation Carbohydrates Cell proliferation Cytokeratin Energy requirements Epithelial cells Epithelial Cells - immunology Epithelial Cells - metabolism Female Galactose Gene expression Glucose Glucose - metabolism Glucose Transport Proteins, Facilitative - genetics Glucose Transport Proteins, Facilitative - metabolism Glucose Transporter Type 1 - genetics Glucose Transporter Type 1 - metabolism Golgi Apparatus - metabolism Golgi cells Human Physiology Immunohistochemistry K protein Keratin-18 - metabolism Lactalbumin Lactalbumin - metabolism Lactation Lactose Lactose - biosynthesis Lactose synthase Lactose Synthase - metabolism Localization Mammary gland Mammary glands Mammary Glands, Animal - metabolism Membrane proteins Mice Mice, Inbred BALB C mRNA Nutrients Original Article Peptides - metabolism Pregnancy Protein folding Proteins Retinoblastoma-Like Protein p130 - metabolism RNA, Messenger - metabolism Synthesis Time Factors Underpotential deposition Weaning |
| title | GLUT1 and GLUT8 support lactose synthesis in Golgi of murine mammary epithelial cells |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-22T16%3A37%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=GLUT1%20and%20GLUT8%20support%20lactose%20synthesis%20in%20Golgi%20of%20murine%20mammary%20epithelial%20cells&rft.jtitle=Journal%20of%20physiology%20and%20biochemistry&rft.au=Villagr%C3%A1n,%20Marcelo&rft.date=2019-06-01&rft.volume=75&rft.issue=2&rft.spage=209&rft.epage=215&rft.pages=209-215&rft.issn=1138-7548&rft.eissn=1877-8755&rft_id=info:doi/10.1007/s13105-019-00679-3&rft_dat=%3Cproquest_cross%3E2215026377%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c375t-4a7e5c5c73cd01c7ff51e592a34ce524d82beef7e682f9b70ad499672ab7af5a3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2252681086&rft_id=info:pmid/31020623&rfr_iscdi=true |