Non-Specific Porins of Yersinia pseudotuberculosis as Inductors of Experimental Hyperthyroidism in Mice

In vivo experiments showed that antibodies to OmpC and OmpF porins of Yersinia pseudotuberculosis increased thyroxine (T4) level in the blood of experimental animals. The mice were immunized with different antigens: recombinant OmpF porin in a soluble monomeric form, trimers of OmpC and OmpF porins...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2019-04, Vol.166 (6), p.751-753
Main Authors: Portnyagina, O. Yu, Golotin, V. A., Zelepuga, E. A., Khomenko, V. A., Shevchenko, L. S., Novikova, O. D.
Format: Article
Language:English
Subjects:
Antibodies
Antigenic determinants
Antigens
Biomedical and Life Sciences
Biomedicine
Blood
Cell Biology
Electronic components
Hormones
Hyperthyroidism
Immunization
Immunoglobulins
Immunology and Microbiology
Internal Medicine
Laboratory Medicine
Pathology
Porins
Proteins
Pseudotuberculosis
Thyroid diseases
Thyroid hormones
Thyroxine
Trimers
Yersinia pseudotuberculosis
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Summary:In vivo experiments showed that antibodies to OmpC and OmpF porins of Yersinia pseudotuberculosis increased thyroxine (T4) level in the blood of experimental animals. The mice were immunized with different antigens: recombinant OmpF porin in a soluble monomeric form, trimers of OmpC and OmpF porins isolated from the outer membrane, or antibodies to them. The level of thyroxine in the blood of mice immunized with OmpF and OmpC porins increased by 5.47 and 22.3 times, respectively; after immunization with antibodies to these proteins, blood thyroxine increased by 9.28 and 14.29 times. Immunization with recombinant OmpF porin induced no reliable increase in thyroxine level. Hence, the serum to recombinant OmpF porin contains no antibodies specific to conformational antigenic determinants that are present in the protein trimer and, according to our previous findings from molecular docking studies, determine cross-reactions between OmpF porin of Y. pseudotuberculosis and thyroidstimulating hormone receptor.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-019-04433-z