Chrysanthemum indicum extract inhibits NLRP3 and AIM2 inflammasome activation via regulating ASC phosphorylation

• Published in: Journal of ethnopharmacology 2019-07, Vol.239, p.111917-111917, Article 111917
• Main Authors: Yu, Sang-Hyeun, Sun, Xiao, Kim, Myong-Ki, Akther, Mahbuba, Han, Jun-Hyuk, Kim, Tae-Yeon, Jiang, Jun, Kang, Tae-Bong, Lee, Kwang-Ho
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; ASC phosphorylation; CARD Signaling Adaptor Proteins - metabolism; Chrysanthemum; Chrysanthemum indicum; DNA-Binding Proteins - metabolism; Female; Gout - drug therapy; Gout - metabolism; Gout Suppressants - pharmacology; Gout Suppressants - therapeutic use; Inflammasome; Inflammasomes - metabolism; MAP Kinase Kinase 4 - metabolism; Mice, Inbred C57BL; MSU-Induced peritonitis; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; NLRP3; Peritonitis - chemically induced; Peritonitis - drug therapy; Peritonitis - metabolism; Phosphorylation - drug effects; Plant Components, Aerial; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Uric Acid
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2019.111917

Chrysanthemum indicum (C. indicum), a perennial plant, has long been used to treat inflammation-related disorders, such as pneumonia, hypertension, gastritis, and gastroenteritis. The inhibitory effect of C. indicum extract (C.I) on inflammasome activation was investigated to validate its potential in treating inflammation related disorders. LPS-primed bone marrow-derived macrophages (BMDMs) were used to confirm the inhibitory effect of C.I on selective inflammasome activation in vitro. A monosodium urate (MSU)-induced murine peritonitis model was employed to study the effect of C.I in vivo. C.I inhibited activation of NLRP3 and AIM2 inflammasomes, leading to suppression of interleukin-1β secretion in vitro. Further, C.I regulates the phosphorylation of apoptosis-associated speck-like protein containing a CARD (ASC), which could be the main contribution to attenuate these inflammasomes activation. C.I also suppressed secretion of pro-inflammatory cytokines and neutrophils recruitment in MSU-induced murine peritonitis model. This study provides scientific evidence substantiating the traditional use of C. indicum in the treatment of inflammatory diseases, including gout, which is induced by physiologically analogous cause to MSU-induced peritonitis. [Display omitted]