A combined approach of 16S rRNA and a functional marker gene, soxB to reveal the diversity of sulphur-oxidising bacteria in thermal springs

With the advent of new molecular tools, new taxa of sulphur-oxidising bacteria (SOB) in diverse environments are being discovered. However, there is a significant gap of knowledge about the ecology and diversity of SOB in thermal springs. Here, the species diversity and phylogenetic affiliations of...

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Bibliographic Details
Published in:Archives of microbiology 2019-09, Vol.201 (7), p.951-967
Main Authors: Jaffer, Y. D., Purushothaman, C. S., Sanath Kumar, H., Irfan, A. B., Gireesh-Babu, P., Ganie, P. A., Bhat, R. A. H., Vennila, A.
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry
Biodiversity
Biomedical and Life Sciences
Biotechnology
Cell Biology
Ecology
Gene sequencing
Hot springs
Life Sciences
Microbial Ecology
Microbiology
New taxa
Original Paper
Oxidation
Phylogeny
Proteobacteria
rRNA 16S
SoxB protein
Species diversity
Sulfur
Thiosulfates
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Summary:With the advent of new molecular tools, new taxa of sulphur-oxidising bacteria (SOB) in diverse environments are being discovered. However, there is a significant gap of knowledge about the ecology and diversity of SOB in thermal springs. Here, the species diversity and phylogenetic affiliations of SOB were investigated using 16S rRNA and functional gene marker, soxB in thermal springs of Thane district of Maharashtra, India. Most SOB detected by 16S rDNA sequences belong to different operational taxonomic units (OTU’s): Firmicutes , α -, β -, γ - Proteobacteria and Actinobacteria with the dominance of first class. However, the sox B gene clone library sequences had shown affiliation with the β -, γ - and α - Proteobacteria . β - Proteobacteria -related sequences were dominant, with 53.3% clones belonging to genus Hydrogenophaga . The thiosulphate oxidation assay carried out for different isolates having distinct identity showed the mean sulphate-sulphur production from 117.86 ± 0.50 to 218.82 ± 2.56 mg SO 4 -S l −1 after 9 days of incubation. Also, sulphur oxidation by the genus Nitratireductor , Caldimonas , Geobacillus , Paenibacillus , Brevibacillus , Tristrella and Chelatococcus has been reported for the first time that reveals ecological widening over which thiotrophs are distributed.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-019-01666-4