Proteomic analysis of calcium-enriched sol–gel biomaterials

Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue–material interface, affecting the protein deposition process and cell re...

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Bibliographic Details
Published in:Journal of biological inorganic chemistry 2019-06, Vol.24 (4), p.563-574
Main Authors: Romero-Gavilán, F., Araújo-Gomes, Nuno, Cerqueira, A., García-Arnáez, I., Martínez-Ramos, C., Azkargorta, M., Iloro, I., Elortza, F., Gurruchaga, M., Suay, J., Goñi, I.
Format: Article
Language:English
Subjects:
Apolipoprotein E
Biochemistry
Biomedical and Life Sciences
Biomedical materials
Blood coagulation
Bone biomaterials
Bone turnover
Calcium chloride
Cell lines
Clotting
Inflammation
Inorganic chemistry
Life Sciences
Microbiology
Original Paper
Proteins
Silica
Tissue engineering
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Summary:Calcium is an element widely used in the development of biomaterials for bone tissue engineering as it plays important roles in bone metabolism and blood coagulation. The Ca ions can condition the microenvironment at the tissue–material interface, affecting the protein deposition process and cell responses. The aim of this study was to analyze the changes in the patterns of protein adsorption on the silica hybrid biomaterials supplemented with different amounts of CaCl 2 , which can function as release vehicles. This characterization was carried out by incubating the Ca-biomaterials with human serum. LC–MS/MS analysis was used to characterize the adsorbed protein layers and compile a list of proteins whose affinity for the surfaces might depend on the CaCl 2 content. The attachment of pro- and anti-clotting proteins, such as THRB, ANT3, and PROC, increased significantly on the Ca-materials. Similarly, VTNC and APOE, proteins directly involved on osteogenic processes, attached preferentially to these surfaces. To assess correlations with the proteomic data, these formulations were tested in vitro regarding their osteogenic and inflammatory potential, employing MC3T3-E1 and RAW 264.7 cell lines, respectively. The results confirmed a Ca dose-dependent osteogenic and inflammatory behavior of the materials employed, in accordance with the protein attachment patterns. Graphical Abstract
ISSN:0949-8257
1432-1327
DOI:10.1007/s00775-019-01662-5