Conductive hydrogel based on chitosan-aniline pentamer/gelatin/agarose significantly promoted motor neuron-like cells differentiation of human olfactory ecto-mesenchymal stem cells

• Published in: Materials Science & Engineering C 2019-08, Vol.101, p.243-253
• Main Authors: Bagher, Zohreh, Atoufi, Zhaleh, Alizadeh, Rafieh, Farhadi, Mohammad, Zarrintaj, Payam, Moroni, Lorenzo, Setayeshmehr, Mohsen, Komeili, Ali, Kamrava, S. Kamran
• Format: Article
• Language: English
• Subjects: Aniline; Aniline Compounds - chemical synthesis; Aniline Compounds - pharmacology; Aniline pentamer; Calcium Phosphates - pharmacology; Cell culture; Cell differentiation; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Cells, Cultured; Chitosan; Chitosan - chemical synthesis; Chitosan - chemistry; Chitosan - pharmacology; Compressive Strength; Conductive polymers; Differentiation (biology); Electric Conductivity; Gelatin; Gelatin - chemistry; Gelatin - pharmacology; Humans; Hydrogels; Hydrogels - chemistry; Hydrogels - pharmacology; Ion currents; Islet-1 protein; Male; Materials science; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - ultrastructure; Mesenchyme; Motor neuron differentiation; Motor neurons; Motor Neurons - cytology; Motor Neurons - drug effects; mRNA; Mucosa; Nasal ectomesenchymal stem cells; Neural crest; Neurons; Olfactory Bulb - cytology; Olfactory epithelium; Organic chemistry; Plates (structural members); Protein biosynthesis; Protein synthesis; RNA, Messenger - genetics; RNA, Messenger - metabolism; Scaffolds; Sepharose - pharmacology; Spectroscopy, Fourier Transform Infrared; Stem cells; Temperature; Thermal properties; Thermodynamic properties; Thermogravimetry; Tissue culture; Tissue engineering; Tissue Scaffolds - chemistry; Transcription
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.03.068

Developing a simple produces for efficient derivation of motor neurons (MNs) is essential for neural tissue engineering studies. Stem cells with high capacity for neural differentiation and scaffolds with the potential to promote motor neurons differentiation are promising candidates for neural tissue engineering. Recently, human olfactory ecto-mesenchymal stem cells (OE-MSCs), which are isolated easily from the olfactory mucosa, are considered a new hope for neuronal replacement due to their neural crest origin. Herein, we synthesized conducting hydrogels using different concentration of chitosan-g-aniline pentamer, gelatin, and agarose. The chemical structures, swelling and deswelling ratio, ionic conductivity and thermal properties of the hydrogel were characterized. Scaffolds with 10% chitosan-g-aniline pentamer/gelatin (S10) were chosen for further investigation and the potential of OE-MSCs as a new source for programming to motor neuron-like cells investigated on tissue culture plate (TCP) and conductive hydrogels. Cell differentiation was evaluated at the level of mRNA and protein synthesis and indicated that conductive hydrogels significantly increased the markers related to motor neurons including Hb-9, Islet-1 and ChAT compared to TCP. Taken together, the results suggest that OE-MSCs would be successfully differentiated into motor neuron-like cells on conductive hydrogels and would have a promising potential for treating motor neuron-related diseases. [Display omitted] •Olfactory ecto-mesenchymal stem cells (OE-MSCs) have tendency to give rise to motor neuron.•Conductive hydrogels has the potential to enhance the motor neuron-like cells differentiation.•Conductive scaffolds containing OE-MSCs holds a new hope for treatment of motor neuron diseases.