DDC plays vital roles in the wing spot formation, egg production, and chorion tanning in the brown planthopper

The gene dopa decarboxylase (Nlddc) of the brown planthopper (BPH, Nilaparvata lugens) was identified in the genome and transcriptome of the insect. The open reading frame of Nlddc is 1,434 bp in length and, it has the potential to encode a protein of 477 amino acid with a conserved pyridoxal‐depend...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2019-06, Vol.101 (2), p.e21552-n/a
Main Authors: Lu, Jia‐Bao, Zhang, Meng‐Qiu, Li, Ling‐Chen, Zhang, Chuan‐Xi
Format: Article
Language:English
Subjects:
Amino acids
Aromatic-L-amino-acid decarboxylase
Brain
Chorion
chorion tanning
Dihydroxyphenylalanine
Egg production
Gene expression
Genomes
Insects
Integument
Nilaparvata lugens (BPH)
Nlddc
Periodicity
Polymerase chain reaction
RNA-mediated interference
Tanning
Transcription
wing spot
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Summary:The gene dopa decarboxylase (Nlddc) of the brown planthopper (BPH, Nilaparvata lugens) was identified in the genome and transcriptome of the insect. The open reading frame of Nlddc is 1,434 bp in length and, it has the potential to encode a protein of 477 amino acid with a conserved pyridoxal‐dependent decarboxylase domain and a typical motif, NFNPHKW. Real‐time quantification polymerase chain reaction analyses revealed that this gene was highly expressed in the integument and brain, and transcript level peaked in the late stages of egg period and each nymph instar with periodicity. RNA interference results revealed that Nlddc played essential roles in pigment synthesis, formation of wing spot, egg production, and tanning of the chorion. A rapid accumulation of Nlddc transcripts was detected, and it coincided with the injection of the hormone 20‐hydroxyecdysone (20E), suggesting that Nlddc transcription was regulated by 20E. D2: adults 2 days after eclosion after treatment with dsNlddc, the wing spots (indicated with red circles) disappeared when compared with the control ones treated with dsGFP. 10min: comparison of dehydration for eggs at 10 minutes after being dissected from the rice tissues between dsNlddc and dsGFP treated groups.
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.21552