Virulence evaluation of classical swine fever virus subgenotype 2.1 and 2.2 isolates circulating in China

• Published in: Veterinary microbiology 2019-05, Vol.232, p.114-120
• Main Authors: Gong, Wenjie, Li, Junhui, Wang, Zunbao, Sun, Jiumeng, Mi, Shijiang, Lu, Zongji, Cao, Jian, Dou, Zhihua, Sun, Yanjun, Wang, Pengjiang, Yuan, Ke, Zhang, Liying, Zhou, Xubin, He, Sun, Tu, Changchun
• Format: Article
• Language: English
• Subjects: Animals; Anorexia; Colostrum; Conjunctivitis; Constipation; CSFV; Dyspnea; Edema; Epidemics; Evaluation; Experimental infection; Exudation; Fever; Field isolate; Gait; Genotypes; Hemorrhage; Hog cholera; Hogs; Lesions; Leukopenia; Livestock; Lymph nodes; Outbreaks; Pathogenicity; Respiration; Swine; Vaccines; Viremia; Virulence; Viruses
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2019.04.001

•Four current and one earlier CSFV field isolates in China were classified as either highly or moderately virulent strains.•E2 and Erns antibodies in infected pigs were generally inadequate for protection, or even absent in some infected pigs.•Viremia and Erns protein were present and long-lasting in the blood of all infected animals. Classical swine fever (CSF) remains an important pig disease in China, where it usually presents with mild or atypical clinical manifestations, with large scale outbreaks rarely seen. This has led to speculation about the possible circulation of viral strains of low virulence. To investigate this possibility, five field isolates within the predominant genotype 2 (2.1b, 2.1c, 2.1 h and 2.2) were evaluated and compared by experimental infection of naturally farrowed but colostrum-deprived piglets. All infected piglets displayed clinical signs, including persistent high fever, depression, anorexia, dyspnea, conjunctivitis, constipation, and hesitant gait. Typical pathological lesions, including pulmonary edema, hemorrhagic or cellulosic exudation, and swelling and hemorrhage of lymph nodes, were observed. Viremia and Erns protein expression in the blood of all infected animals were detectable from 3 to 5 days post infection (DPI), their presence correlating with the onset of fever, clinical signs and leukopenia. E2 antibody did not develop in any of the field CSFV-infected piglets during the disease course, while Erns antibody was detectable in 4–56% of infected animals at various time points. Mortalities ranged from 20 to 80% within 21 DPI, progressing to 100% by 43 DPI. Based on clinical scores and fatalities within 21 DPI, 2 of the 5 field isolates were classified as of moderate virulence and 3 of high virulence; i.e., no field isolates of low virulence were identified. The study has provided data supporting the use of these isolates as challenge viruses to evaluate the efficacy of current CSF vaccines.