Antiproliferative effects of new magnetic pH-responsive drug delivery system composed of Fe3O4, CaAl layered double hydroxide and levodopa on melanoma cancer cells

• Published in: Materials Science & Engineering C 2019-08, Vol.101, p.472-486
• Main Authors: Shahabadi, Nahid, Razlansari, Mahtab, Zhaleh, Hossein, Mansouri, Kamran
• Format: Article
• Language: English
• Subjects: Anticancer properties; Antitumor activity; Cancer; Cell culture; Cell death; Colorimetry; Core-shell structure; Cytotoxicity; Dihydroxyphenylalanine; Drug delivery; Drug delivery systems; Drug release; Encapsulation; Hydrogen bonding; Hydrogen bonds; Hydroxides; Iron oxides; l-Dopa; Layered double hydroxide; Levodopa; Magnetic nano carrier; Materials science; Melanoma; Nanoparticles; Toxicity; X ray photoelectron spectroscopy; Zeta potential
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2019.04.004

In this study, an efficient drug delivery system composed of Fe3O4, CaAl layered double hydroxide (LDH) and l-Dopa has been synthesized through hydrogen bonds between l-Dopa and CaAl-LDH encapsulated Fe3O4 nanoparticles (Fe3O4@CaAl-LDH@l-Dopa). The structural features of Fe3O4@CaAl-LDH@l-Dopa were characterized using XRD, SEM, TEM, EDX, FT-IR, VSM, TGA, XPS, zeta potential analysis and BET. All of the characterization techniques show the uniform high surface area core-shell structure with about 120 nm in average size. Also, the obtained results clearly indicate that this drug delivery system possess high potent for adsorption of l-Dopa (52 wt%) and high drug encapsulation efficiency (71%). The amount of l-Dopa release in low pHs (53.8%) which simulates the environment of cancer cells is greater than higher pHs. The in vitro cytotoxic and anticancer activities of Fe3O4@CaAl-LDH@l-Dopa were investigated against Mel-Rm Cells Melanoma (NCIt: C3224) using LDH colorimetric assay and differential staining cell death assay. The results showed Fe3O4@CaAl-LDH@l-Dopa with a lower concentration of l-Dopa, illustrate a higher cytotoxicity and anticancer activity. [Display omitted] •An efficient drug delivery system composed of Fe3O4, CaAl layered double hydroxide (LDH) and l-Dopa has been synthesized.•The uniform high surface area core-shell structure with about 120 nm in average size was observed.•The amount of l-Dopa release in low pHs (53.8%) which simulates the environment of cancer cells is greater than higher pHs.•Fe3O4@CaAl-LDH@l-Dopa with a lower concentration of l-Dopa illustrates a higher cytotoxicity and anticancer activity.