Paper surface modification strategies employing N-SBA-15/polymer composites in bioanalytical sensor design

• Published in: Talanta (Oxford) 2019-08, Vol.200, p.186-192
• Main Authors: Moreira, Cristian M., Scala-Benuzzi, Maria L., Takara, Eduardo A., Pereira, Sirley V., Regiart, Matías, Soler-Illia, Galo J.A.A., Raba, Julio, Messina, Germán A.
• Format: Article
• Language: English
• Subjects: Adsorption; Ascorbic Acid - analysis; Ascorbic acid, N-SBA-15; Fluorescence; Horseradish Peroxidase - chemistry; Horseradish Peroxidase - metabolism; Hydrogen Peroxide - chemistry; Nitrogen - chemistry; Paper-based device; Particle Size; Polyethylenimine; Polymers - chemistry; Silicon Dioxide - chemical synthesis; Silicon Dioxide - chemistry; Spectroscopy, Fourier Transform Infrared; Surface Properties
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2019.03.051

In this work, different paper surface modification strategies were compared to obtain an amine functionalized SBA-15 (N-SBA-15) composite for paper-based device development. The synthesized N-SBA-15 was characterized by N2 adsorption-desorption isotherm, and infrared spectroscopy (FTIR), and it was incorporated to different polymer matrices (κ-carrageenan (CA), polyvinyl alcohol (PVA) and polyethylenimine (PEI)) for the development of the composite modified paper-based device. The retention, interactions, and morphology of the obtained composites were investigated by absorbance measurement, FTIR and scanning electron microscopy (SEM), respectively. To demonstrate the applicability of the modified paper-based device, ascorbic acid (AA) quantification was carried out. Horseradish peroxidase (HRP) was immobilized onto the modified paper surface. HRP in the presence of H2O2 catalyzes the oxidation of 10-acetyl-3,7-dyhidroxyphenoxazine (ADHP) to highly fluorescent resorufin, which was measured by LIF detector. Thus, when AA was added to the solution, it decreases the relative fluorescence signal proportionally to the AA concentration. The linear range from 50 nmol L−1 to 1500 nmol L−1 and a detection limit of 15 nmol L−1 were obtained for AA quantitation. The obtained results allowed us to conclude that N-SBA-15/PEI composite could be considered an excellent choice for the paper-based device modification procedure due to its inherent simplicity, low cost, and sensitivity. [Display omitted] •Different modification strategies are compared to obtain an amine functionalized SBA-15 composite modified paper device.•N-SBA-15/PEI composite shows the highest signal response, providing an excellent platform for the biosensors construction.•This device represents a novel strategy for the paper-based biosensor development.