Plant regeneration from in vitro-cultured seedling leaf protoplasts of Actinidia eriantha Benth

• Published in: Plant cell reports 1998-07, Vol.17 (10), p.819-821
• Main Authors: Zhang, Y.J, Qian, Y.Q, Mu, X.J, Cai, Q.G, Zhou, Y.L, Wei, X.P
• Format: Article
• Language: English
• Subjects: 2,4-D; 2-(n-morpholino)ethanesulphonic acid; Acetic acid; Actinidia; Actinidia eriantha; benzyladenine; Biological and medical sciences; Biotechnology; callus; casein hydrolysate; chromosome number; culture media; dose response; Eukaryotic cell cultures; explants; Fundamental and applied biological sciences. Psychology; gibberellic acid; glucose; Growth regulators; in vitro culture; indole acetic acid; indole butyric acid; Leaves; methodology; Methods. Procedures. Technologies; micropropagation; naphthaleneacetic acid; nuclei; Plant cells and fungal cells; Protoplast preparation, cell fusion and regeneration; protoplasts; quantitative analysis; regenerative ability; Seedlings; Shoots; somaclonal variation; zeatin
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050490

Newly expanded in vitro leaves of Actinidia eriantha were used for protoplast isolation. Protoplasts were cultured in liquid MS medium (lacking NH4NO3) supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.4 M glucose. The plating efficiency after 3 weeks of culture was 19.4%, and calli were recovered without addition of fresh medium. These calli regenerated shoots on transfer to MS medium containing 2.28 micromolar zeatin and 0.57 micromolar IAA (indole-3-acetic acid). Regenerated shoots were rooted by immersion in 20 ppm IBA (indole-3-butyric acid) solution before culturing on half-strength MS medium lacking growth regulators. Somaclonal variation, in terms of chromosome number and nuclei per cell of protoplast-derived plants, was estimated.