Cryopreservation of embryogenic tissue of a range of genotypes of sweet potato (Ipomoea batatas [L.] Lam.) using an encapsulation protocol

Embryogenic tissue of nine sweet potato [Ipomoea batatas (L.) Lam] genotypes from Asia, Africa and the Americas was established from in vitro axillary buds on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. Embryogenic aggregates, 1.0...

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Bibliographic Details
Published in:Plant cell reports 1997-09, Vol.16 (11), p.802-806
Main Authors: Bhatti, M.H, Percival, T, Davey, C.D.M, Henshaw, G.G, Blakesley, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
cryopreservation
Dehydration
dehydration (physiological)
embryo (plant)
encapsulation
Eukaryotic cell cultures
Freezing
Fundamental and applied biological sciences. Psychology
genetic variation
genotype
Genotypes
germplasm
In vitro embryo culture
Ipomoea batatas
methodology
Methods. Procedures. Technologies
Plant cells and fungal cells
Potatoes
pretreatment
somatic embryogenesis
sucrose
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Summary:Embryogenic tissue of nine sweet potato [Ipomoea batatas (L.) Lam] genotypes from Asia, Africa and the Americas was established from in vitro axillary buds on Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic acid or 2,4,5-trichlorophenoxyacetic acid. Embryogenic aggregates, 1.0-2.0 mm in diameter, were encapsulated in alginate gel, precultured on medium containing elevated levels of sucrose and dehydrated prior to rapid freezing in liquid nitrogen. The maximum survival of embryogenic tissue ranged from 4% to 38%, depending on the genotype. With the incorporation of a slow-cooling step, survival was generally much higher than that obtained after rapid freezing alone. Five of eight genotypes tested with this protocol gave survival percentages in excess of 55%, and a further two in excess of 33%, all after evaporative dehydration. The most effective sucrose treatment(s), however, varied with the genotype.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050324