Tissue culture of wild-type, interspecific genome/plastome hybrids and plastome mutants of evening primrose (Oenothera): controlled morphogenesis and transformation

A favourable combination of genetic features in the genus Oenothera offers access to fundamental biological aspects that are not readily approached with other materials. We have developed protocols for cell and tissue culture as well as for transformation, in order to establish the basis for a compr...

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Bibliographic Details
Published in:Plant cell reports 1998-05, Vol.17 (8), p.605-611
Main Authors: Mehra-Palta, A, Koop, H.U, Goes, S, Troidl, E.M, Nagy, G, Tyagi, S, Kofer, W, Herrmann, R.G
Format: Article
Language:English
Subjects:
2,4-D
Acetic acid
Agrobacterium tumefaciens
benzyladenine
Biological and medical sciences
Biotechnology
callus
cell biology
culture media
developmental stages
dose response
explants
Fundamental and applied biological sciences. Psychology
gene transfer
Genetic engineering
Genetic technics
genetic transformation
genetic variation
Genomes
genotype
Genotypes
gibberellic acid
growth
Hybrids
indole acetic acid
indole butyric acid
kinetin
leaves
methodology
Methods. Procedures. Technologies
micropropagation
Molecular biology
murashige and skoog medium
mutants
nagata and takebe medium
naphthaleneacetic acid
Oenothera
oenothera ammophila
oenothera argillicola
Oenothera biennis
oenothera grandiflora
oenothera hookeri
oenothera parviflora
plant morphology
regenerative ability
Seedlings
shoots
tissue culture
Transgenic animals and transgenic plants
Transgenic plants
wild strains
zeatin
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Summary:A favourable combination of genetic features in the genus Oenothera offers access to fundamental biological aspects that are not readily approached with other materials. We have developed protocols for cell and tissue culture as well as for transformation, in order to establish the basis for a comprehensive cell and molecular biology of Euoenothera species, their genome/plastome hybrids and plastome mutants. Regeneration of plants from excised seedling parts (roots, hypocotyl, cotyledons, shoot tips) and leaf explants was optimal on NT medium containing 1 mg(.)l-1 6-benzylaminopurine and 3 mg(.)l-1 alpha-naphtalene acetic acid. This medium also proved to be efficient in the propagation of various wild-type genotypes, interspecific hybrids and plastome mutants. Using Ti-based approaches we also succeeded in generating transgenic Oenothera plants with relatively high efficiency.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050451