RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var. indica) somaclonal progenies

RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar,indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphi...

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Bibliographic Details
Published in:Plant cell reports 1997-02, Vol.16 (5), p.320-324
Main Authors: GODWIN, I. D, SANGDUEN, N, KUNANUVATCHAIDACH, R, PIPERIDIS, G, ADKINS, S. W
Format: Article
Language:English
Subjects:
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Cultivars
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Mutation
Oryza sativa indica
Pteridophyta, spermatophyta
Vegetals
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Summary:RAPD analysis was performed among eight rice somaclonal families known to vary for specific characters and four somaclonal families which were phenotypically normal. The parental cultivar,indica rice cv. FR13A, was found to be homogeneous and homozygous at all but one of the 45 RAPD loci. Polymorphisms were found at 28 of the 45 bands among the somaclonal families, including both loss of parental bands, and the appearance of novel non-parental bands. Segregation data revealed both heterozygous and homozygous mutation events, with recessive mutations more prevalent than dominant. All somaclonal families differed significantly from the parental material, indicating that genomic alterations occurred in all families regardless of phenotype. None of the variant families could be regarded as isogenic lines of FR13A at the DNA level. However, some of the DNA level variation may be in highly repeated sequences with no phenotypic effects. The implications for somaclonal breeding and genetic engineering programs are discussed.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF01088289