Transformation of lisianthus (Eustoma grandiflorum)

Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase I...

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Bibliographic Details
Published in:Plant cell reports 1997-10, Vol.16 (12), p.853-858
Main Authors: LEDGER, S. E, DEROLES, S. C, MANSON, D. G, BRADLEY, J. M, GIVEN, N. K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Cultivars
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic technics
Leaves
Methods. Procedures. Technologies
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Transformed plants from three cultivars of Eustoma grandiflorum (lisianthus) were produced by cocultivating young leaf pieces with Agrobacterium tumefaciens strain A722 containing the binary vectors pKIWI110 and pLN26. Both vectors contain the selectable marker gene for neomycin phosphotransferase II. pKIWI110 also contains the reporter gene for β-D-glucuronidase, and pLN26, the chalcone synthase antisense gene. Southern DNA analysis revealed that all the kanamycin-resistant transformants tested contained copies of the transgenes integrated in their genome. The two plants transformed with pKIWI110 show β-D-glucuronidase expression in their mature leaves and selected transformants passed on the kanamycin-resistant phenotype to the F1 generation.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050333