Strategies for the improvement of salidroside production in cell suspension cultures of Rhodiola sachalinensis

Strategies of elicitation and precursor feeding were applied to improve salidroside production in cell suspension cultures of Rhodiola sachalinensis. Of the seven elicitors examined, that extracted from Aspergillus niger was the most effective, increasing the salidroside content by five-fold when ad...

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Bibliographic Details
Published in:Plant cell reports 1998-02, Vol.17 (4), p.288-293
Main Authors: Xu, J.F, Liu, C.B, Han, A.M, Feng, P.S, Su, Z.G
Format: Article
Language:English
Subjects:
Aspergillus niger
Biological and medical sciences
biosynthesis
Biotechnology
cell suspension culture
culture media
dose response
drugs
elicitors
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
glucosides
induction
Methods. Procedures. Technologies
Miscellaneous
phenolic compounds
phenylalanine
Plant cells and fungal cells
plant extracts
precursors
quantitative analysis
Rhodiola
Rhodiola sachalinensis
tyrosine
tyrosol
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Summary:Strategies of elicitation and precursor feeding were applied to improve salidroside production in cell suspension cultures of Rhodiola sachalinensis. Of the seven elicitors examined, that extracted from Aspergillus niger was the most effective, increasing the salidroside content by five-fold when added on the day of inoculation 40 mg carbohydrate is medium. Three possible precursors for salidroside synthesis, L-phenylalanine, L-tyrosol and L-tyrosine were added to the cultures. A high content of salidroside (1.440%) was attained with an initial L-tyrosol concentration of 0.5 mM in the medium. Combined application of the two strategies resulted in a significantly high salidroside content of 1.734%, corresponding to a salidroside yield of 200 mg/l.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050394