Comparisons of the efficiency of some promoters in silver birch (Betula pendula)

The efficiency of several promoters (pin2 from potato, ubiquitin from sunflower, rolC from Agrobacterium rhizogenes, act1 from rice and CaMV 35S from cauliflower mosaic virus) fused to the uidA reporter gene was measured after biolistic bombardment of birch leaves (Betula pendula L.). The highest le...

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Bibliographic Details
Published in:Plant cell reports 1998-03, Vol.17 (5), p.356-361
Main Authors: Keinonen-Mettala, K, Pappinen, A, Weissenberg, K. von
Format: Article
Language:English
Subjects:
act1 genes
Agrobacterium rhizogenes
beta-glucuronidase
Betula pendula
Biological and medical sciences
Biotechnology
camv 35s genes
Cauliflower mosaic virus
enzyme activity
Fundamental and applied biological sciences. Psychology
gene expression
gene transfer
Genetic engineering
Genetic technics
genetic transformation
insect pests
leaves
methodology
Methods. Procedures. Technologies
Modification of gene expression level
pest resistance
pin2 genes
plant breeding
plasmids
Potatoes
promoter regions
reporter genes
rolc genes
roots
stems
transgenic plants
ubiquitin genes
uida genes
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Summary:The efficiency of several promoters (pin2 from potato, ubiquitin from sunflower, rolC from Agrobacterium rhizogenes, act1 from rice and CaMV 35S from cauliflower mosaic virus) fused to the uidA reporter gene was measured after biolistic bombardment of birch leaves (Betula pendula L.). The highest level of beta-glucuronidase (GUS) activity was achieved with the pin2 promoter and the lowest activity with the CaMV 35S promoter. The activity of the potato wound-inducible promoter (pin2) was also tested in stably transformed birch. The promoter showed induced activity after mechanical wounding and feeding by leaf weevils. The systemic effect was confirmed by enhanced GUS activity in non-wounded leaves. The results of this study indicated that the potato wound-inducible promoter maintains its function in birch and would be a suitable promoter in studies of insect-birch interaction at the molecular level.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050406