simple system for pea transformation

The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported in that it uses dry seed as starting material, and...

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Bibliographic Details
Published in:Plant cell reports 1997-05, Vol.16 (8), p.513-519
Main Authors: Bean, S.J, Gooding, P.S, Mullineaux, P.M, Davies, D.R
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Biological and medical sciences
Biotechnology
cotyledons
developmental stages
fertility
Fundamental and applied biological sciences. Psychology
gene transfer
Genetic engineering
Genetic technics
genetic transformation
meristems
methodology
Methods. Procedures. Technologies
micropropagation
phenotype
Pisum sativum
plant morphology
rapid methods
regenerative ability
shoots
Transgenic animals and transgenic plants
Transgenic plants
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Summary:The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated transformation system. This procedure exhibits distinct advantages over those previously reported in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
ISSN:0721-7714
1432-203X
DOI:10.1007/BF01142315