Protocol for in vitro propagation of Excoecaria agallocha L., a medicinally important mangrove species

An in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node of binodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on...

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Bibliographic Details
Published in:Plant cell reports 1998-08, Vol.17 (11), p.861-865
Main Authors: Rao, C.S, Eganathan, P, Anand, A, Balakrishna, P, Reddy, T.P
Format: Article
Language:English
Subjects:
benzyladenine
biochemical techniques
Biological and medical sciences
Biotechnology
chemical composition
culture media
dosage
Eukaryotic cell cultures
Excoecaria agallocha
explants
Fundamental and applied biological sciences. Psychology
growth
In vitro propagation: entire plant regeneration from tissues and cell cultures
indole butyric acid
medicinal plants
Methods. Procedures. Technologies
Micronutrients
micropropagation
mortality
Natural environment
Plant cells and fungal cells
plant cuttings
Plant species
regenerative ability
Seedlings
Shoots
sprouting
tissue culture
Trees
zeatin
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Summary:An in vitro propagation protocol has been developed for Excoecaria agallocha L. (Euphorbiaceae), a mangrove species. Nodal segments were used for axillary shoot proliferation. One shoot from each node of binodal explants was observed 3 weeks after inoculation. The best axillary sprouting was seen on a newly formulated medium containing BA, Zeatin and IBA in concentrations of 13.3 micromolar, 4.65 micromolar and 1.23 micromolar, respectively. The new medium, first used in this study, has a specific composition of major nutrients, MS micronutrients and iron compounds. Nodal segments from rooted cuttings and seedlings responded better than those of mature tree explants. Multiple shoot induction was complemented with efficient shoot elongation, and repeated subculture of binodal segments from axillary shoots resulted in 10-12 shoots per explant in 3 months. Rooting was achieved by growing shoots in the new medium with 0.23 micromolar IBA. Regenerated plants were successfully acclimatized to the natural environment, and about 85% of plantlets survived under ex vitro conditions. This is the first report of micropropagation in the genus Excoecaria and also in mangrove tree species.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050498