Use of green fluorescent protein for detection of transformed shoots and homozygous offspring

Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds a...

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Bibliographic Details
Published in:Plant cell reports 2000-01, Vol.19 (3), p.219-223
Main Authors: MOLINIER, J, HIMBER, C, HAHNE, G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Nicotiana tabacum
Offspring
Proteins
Seedlings
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Using tobacco as a model system, the data obtained demonstrated that the green fluorescent protein (GFP) can be used as a visual selection marker for transformed tissues. Based on differences in the intensity of GFP fluorescence, homozygous and hemizygous states could be easily visualized in seeds and seedlings of the T1 generation. These results were confirmed by genetic analysis. Optimized conditions for GFP analysis of stable transformants are presented.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050002