Regulation of polyphenol production in Vitis vinifera cell suspension cultures by sugars

Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 M sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars were required fo...

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Bibliographic Details
Published in:Plant cell reports 1998-09, Vol.17 (12), p.946-950
Main Authors: Larronde, F, Krisa, S, Decendit, A, Cheze, C, Deffieux, G, Merillon, J.M
Format: Article
Language:English
Subjects:
Accumulation
Agronomy. Soil science and plant productions
anthocyanins
Biological and medical sciences
biosynthesis
cell growth
cell suspension culture
Cells
chemical constituents of plants
culture media
dose response
Economic plant physiology
fructose
Fundamental and applied biological sciences. Psychology
glucose
mannitol
Metabolism
Metabolism. Physicochemical requirements
Nitrogen metabolism and other ones (excepting carbon metabolism)
Nutrition. Photosynthesis. Respiration. Metabolism
Plant physiology and development
polyphenols
quantitative analysis
Regulation
sorbitol
stilbenes
Sucrose
Sugar
Vitis vinifera
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Summary:Sucrose was found to modulate polyphenol accumulation in Vitis vinifera cell cultures. The production of anthocyanins increased 12-fold after addition of 0.15 M sucrose, while that of stilbenes was only slightly affected. Sucrose did not play a physical role because metabolic sugars were required for the induction of polyphenol accumulation. Indeed, the polyols, mannitol and sorbitol, had no effect on this accumulation. We established a model system to investigate the mechanism of sucrose regulation of polyphenol production without inhibition of grape cell growth. After addition of sucrose to the culture medium, the major sugars accumulated in grape cells were glucose and fructose, reaching 40% of the dry weight. The increase in the level of these hexoses closely coincided with the increase in anthocyanin accumulation in grape cells.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050515