Cryopreservation of Doritaenopsis suspension culture by vitrification

Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-t...

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Bibliographic Details
Published in:Plant cell reports 2000-12, Vol.19 (12), p.1160-1164
Main Authors: TSUKAZAKI, H, MII, M, TOKUHARA, K, ISHIKAWA, K
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Dehydration
Doritaenopsis
Establishment of new cell lines, improvement of cultural methods, mass culture
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Plant cells and fungal cells
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Summary:Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid New Dogashima medium with 0.1 M sucrose and 1.0 mg/l abscisic acid for 1 week at 25  °C in the light. Dehydration by PVS2 was important for the cryopreservation of Doritaenopsis cells. Protocorm-like bodies were induced from cryopreserved cells without morphological variations.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990000255