Genome sequence of a spore-laccase forming, BPA-degrading Bacillus sp. GZB isolated from an electronic-waste recycling site reveals insights into BPA degradation pathways

Bisphenol A (BPA) is a synthetic chemical with known deleterious effects on biota. A genome sequencing project is an important starting point for designing a suitable BPA bioremediation process, because it provides valuable genomic information about the physiological, metabolic, and genetic potentia...

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Bibliographic Details
Published in:Archives of microbiology 2019-07, Vol.201 (5), p.623-638
Main Authors: Das, Ranjit, Liang, Zhishu, Li, Guiying, Mai, Bixian, An, Taicheng
Format: Article
Language:English
Subjects:
Bacillus
Biochemistry
Biomedical and Life Sciences
Bioremediation
Biota
Biotechnology
Bisphenol A
Cell Biology
Comparative studies
Degradation
Dismantling
Ecology
Electronic waste
Gene sequencing
Genes
Genomes
Laccase
Life Sciences
Microbial Ecology
Microbiology
Nucleotide sequence
Organic chemistry
OriginalPaper
Polluted environments
Proteins
rRNA
tRNA
Waste recycling
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Summary:Bisphenol A (BPA) is a synthetic chemical with known deleterious effects on biota. A genome sequencing project is an important starting point for designing a suitable BPA bioremediation process, because it provides valuable genomic information about the physiological, metabolic, and genetic potential of the microbes used for the treatment. This study explored genomic insights provided by the BPA-degrading strain Bacillus sp. GZB, previously isolated from electronic-waste-dismantling site. The GZB genome is a circular chromosome, comprised of a total of 4,077,007 bp with G+C content comprising 46.2%. Genome contained 23 contigs encoded by 3881 protein-coding genes with nine rRNA and 53 tRNA genes. A comparative study demonstrated that strain GZB bloomed with some potential features as compared to other Bacillus species. In addition, strain GZB developed spore cells and displayed laccase activity while growing at elevated stress levels. Most importantly, strain GZB contained many protein-coding genes associated with BPA degradation, as well as the degradation of several other compounds. The protein-coding genes in the genome revealed the genetic mechanisms associated with the BPA degradation by strain GZB. This study predicts four possible degradation pathways for BPA, contributing to the possible use of strain GZB to remediate different polluted environments in the future.
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-019-01622-2