Adjunctive antimicrobial photodynamic therapy using methylene blue/ethanol formulation in experimental periodontitis in diabetic rats: short-term results

The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes....

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Bibliographic Details
Published in:Lasers in medical science 2019-08, Vol.34 (6), p.1253-1260
Main Authors: Filipini, Silvana Martins Rodrigues, Campagnolo, Cibele Bruno, Dutra, Danilo Antônio Milbradt, Maciel, Roberto Marinho, Danesi, Cristiane Cadermatori, Kantorski, Karla Zanini
Format: Article
Language:English
Subjects:
Animals
Antimicrobial agents
Bone loss
Carboxymethylcellulose
Collagen
Dentistry
Diabetes
Diabetes mellitus
Ethanol
Gum disease
Inflammation
Lasers
Mandible
Medicine
Medicine & Public Health
Methylene blue
Optical Devices
Optics
Original Article
Periodontitis
Photodynamic therapy
Photonics
Planing
Quantum Optics
Rodents
Scaling
Streptozocin
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Summary:The aim of this study was to evaluate the effect of an MB experimental formulation (ethanol 20%) in aPDT used as an adjuvant to scaling and root planing (SRP) in the periodontal treatment of diabetic rats. Forty male Wistar rats received streptozotocin-intraperitonial injections to induce diabetes. After 14 days, 5 animals were allocated in the non-ligate group (NLG), and 35 animals received ligature at the first right mandibular molar to induce periodontitis. After 7 days, the ligature was removed and the animals were randomized into 4 groups: LG (without treatment, n  = 5), SRPG (SRP, n  = 10), aPDTW (SRP+aPDT-MB/water, n  = 10), and aPDTEt (SRP + aPDT-MB/water/ethanol/carboxymethylcellulose, n  = 10). Animals were euthanized after 7 days. Data of bone loss (BL) area, degree of inflammatory cell response, and collagen fibers percentages were statistically analyzed ( p   0.05). Our findings showed aPDTEt presented promising results. aPDT using MB/ethanol can have potential as an adjunctive periodontal treatment in diabetics.
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-019-02733-4