Evaluation of resistance in Osteospermum ecklonis (DC.) Norl. plants transgenic for the N protein gene of tomato spotted wilt virus

The nucleocapsid protein (N) gene of tomato spotted wilt virus (TSWV) was inserted into Osteospermum ecklonis via Agrobacterium tumefaciens leaf strips co-cultivation. Sixteen primary transformant clones of two O. ecklonis genotypes were analysed. Southern blots of restricted genomic DNA demonstrate...

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Bibliographic Details
Published in:Plant cell reports 2000-10, Vol.19 (10), p.983-988
Main Authors: VAIRA, A. M, BERIO, T, ACCOTTO, G. P, VECCHIATI, M, ALLAVENA, A
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
AN gene
Biological and medical sciences
Biotechnology
Cloning
Frankliniella occidentalis
Fundamental and applied biological sciences. Psychology
Gene expression
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Genetic engineering
Genetic technics
Genotypes
Immunization
Methods. Procedures. Technologies
N gene
Osteospermum ecklonis
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Thripidae
Tomato spotted wilt virus
Tomatoes
Transgenic animals and transgenic plants
Transgenic plants
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Summary:The nucleocapsid protein (N) gene of tomato spotted wilt virus (TSWV) was inserted into Osteospermum ecklonis via Agrobacterium tumefaciens leaf strips co-cultivation. Sixteen primary transformant clones of two O. ecklonis genotypes were analysed. Southern blots of restricted genomic DNA demonstrated integration of the transgene and indicated the number of integrated copies. Expression of the transgene was estimated by DAS-ELISA and Western and Northern blotting. Plants were challenged with TSWV inoculation, either mechanically or by the thrips Frankliniella occidentalis; they were then monitored for symptom appearance and tested by TAS-ELISA for infection. Inoculation of the transgenic clones via the natural TSWV vector was more efficient and led to the identification of 1 clone, characterised by multiple transgene integration and no transgene expression, with improved resistance to TSWV.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990000226