Inhibition of fungal growth in planta and in vitro by transgenic tobacco expressing a bacterial nonheme chloroperoxidase gene

Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays of CPO-P enzyme activity. Ex...

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Bibliographic Details
Published in:Plant cell reports 2000-03, Vol.19 (4), p.333-338
Main Authors: RAJASEKARAN, K, CARY, J. W, JACKS, T. J, STROMBERG, K. D, CLEVELAND, T. E
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
anthracnose
Aspergillus flavus
Biological and medical sciences
Biotechnology
chloroperoxidase
Colletotrichum destructivum
CPO-P gene
Disease resistance
Enzymatic activity
Fundamental and applied biological sciences. Psychology
Fungi
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Nicotiana tabacum
Plant diseases
Plant extracts
Pseudomonas pyrrocinia
Tobacco
Transgenic animals and transgenic plants
Transgenic plants
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Summary:Transgenic tobacco plants producing chloroperoxidase (CPO-P), encoded by a novel gene from Pseudomonas pyrrocinia, were obtained by Agrobacterium-mediated transformation. Successful transformation was shown by PCR, Southern, northern and western blot analyses, and assays of CPO-P enzyme activity. Extracts from plants transformed with the CPO-P gene significantly reduced Aspergillus flavus colonies by up to 100% compared with extracts from control plants transformed with pBI121. Compared with controls, the transformed plants showed increased disease resistance in planta against a fungal pathogen, Colletotrichum destructivum, the causal agent of tobacco anthracnose.
ISSN:0721-7714
1432-203X
DOI:10.1007/s002990050736