Adipose Stem Cells Enhance Nerve Regeneration and Muscle Function in a Peroneal Nerve Ablation Model

Severe peripheral nerve injuries have devastating consequences on the quality of life in affected patients, and they represent a significant unmet medical need. Destruction of nerve fibers results in denervation of targeted muscles, which, subsequently, undergo progressive atrophy and loss of functi...

Full description

Saved in:
Bibliographic Details
Published in:Tissue engineering. Part A 2021-03, Vol.27 (5-6), p.297-310
Main Authors: Passipieri, Juliana A, Dienes, Jack, Frank, Joseph, Glazier, Joshua, Portell, Andrew, Venkatesh, Kaushik P, Bliley, Jacqueline M, Grybowski, Damian, Schilling, Benjamin K, Marra, Kacey G, Christ, George J
Format: Article
Language:English
Subjects:
Ablation
Atrophy
Denervation
Homeostasis
Hydrogels
Innervation
Muscle contraction
Muscle function
Original Articles
Peripheral nerves
Peroneal nerve
Polarity
Polycaprolactone
Preservation
Quality of life
Recovery of function
Regeneration
Skeletal muscle
Statistical analysis
Stem cell transplantation
Stem cells
Transplants & implants
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Severe peripheral nerve injuries have devastating consequences on the quality of life in affected patients, and they represent a significant unmet medical need. Destruction of nerve fibers results in denervation of targeted muscles, which, subsequently, undergo progressive atrophy and loss of function. Timely restoration of neural innervation to muscle fibers is crucial to the preservation of muscle homeostasis and function. The goal of this study was to evaluate the impact of addition of adipose stem cells (ASCs) to polycaprolactone (PCL) nerve conduit guides on peripheral nerve repair and functional muscle recovery in the setting of a critical size nerve defect. To this end, peripheral nerve injury was created by surgically ablating 6 mm of the common peroneal nerve in a rat model. A PCL nerve guide, filled with ASCs and/or poloxamer hydrogel, was sutured to the nerve ends. Negative and positive controls included nerve ablation only (no repair), and reversed polarity autograft nerve implant, respectively. Tibialis anterior (TA) muscle function was assessed at 4, 8, and 12 weeks postinjury, and nerve and muscle tissue was retrieved at the 12-week terminal time point. Inclusion of ASCs in the PCL nerve guide elicited statistically significant time-dependent increases in functional recovery (contraction) after denervation; ∼25% higher than observed in acellular (poloxamer-filled) implants and indistinguishable from autograft implants, respectively, at 12 weeks postinjury ( p  
ISSN:1937-3341
1937-335X
DOI:10.1089/ten.tea.2018.0244