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Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus
Cinchona officinalis 'Ledgeriana', former called Cinchona ledgeriana, hairy roots were initiated containing constitutive-expression constructs of cDNAs encoding the enzymes tryptophan decarboxylase (TDC) and strictosidine synthase (STR) from Catharanthus roseus, two key enzymes in terpenoi...
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| Published in: | Plant cell reports 1999-12, Vol.19 (2), p.191-196 |
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| Main Authors: | , , , , , |
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| container_end_page | 196 |
| container_issue | 2 |
| container_start_page | 191 |
| container_title | Plant cell reports |
| container_volume | 19 |
| creator | Geerlings, A Hallard, D Martinez Caballero, A Lopes Cardoso, I van der Heijden, R Verpoorte, R |
| description | Cinchona officinalis 'Ledgeriana', former called Cinchona ledgeriana, hairy roots were initiated containing constitutive-expression constructs of cDNAs encoding the enzymes tryptophan decarboxylase (TDC) and strictosidine synthase (STR) from Catharanthus roseus, two key enzymes in terpenoid indole and quinoline alkaloid biosynthesis. The successful integration of these genes and the reporter gene gus-int was demonstrated using Southern blotting and the polymerase chain reaction. The products of TDC and STR, tryptamine and strictosidine, were found in high amounts, 1200 and 1950 μg g
dry weight, respectively. Quinine and quinidine levels were found to rise up to 500 and 1000 μg g
dry weight, respectively. The results show that genetic engineering with multiple genes is well possible in hairy roots of C. officinalis. However, 1 year after analyzing the hairy roots for the first time, they had completely lost their capacity to accumulate alkaloids. |
| doi_str_mv | 10.1007/s002990050732 |
| format | article |
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dry weight, respectively. Quinine and quinidine levels were found to rise up to 500 and 1000 μg g
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dry weight, respectively. Quinine and quinidine levels were found to rise up to 500 and 1000 μg g
dry weight, respectively. The results show that genetic engineering with multiple genes is well possible in hairy roots of C. officinalis. 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dry weight, respectively. Quinine and quinidine levels were found to rise up to 500 and 1000 μg g
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| fulltext | fulltext |
| identifier | ISSN: 0721-7714 |
| ispartof | Plant cell reports, 1999-12, Vol.19 (2), p.191-196 |
| issn | 0721-7714 1432-203X |
| language | eng |
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| source | Springer Nature |
| subjects | Bacteria Biosynthesis Genes Genetic engineering Roots |
| title | Alkaloid production by a Cinchona officinalis 'Ledgeriana' hairy root culture containing constitutive expression constructs of tryptophan decarboxylase and strictosidine synthase cDNAs from Catharanthus roseus |
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