Streptococcus periodonticum sp. nov., Isolated from Human Subgingival Dental Plaque of Periodontitis Lesion

• Published in: Current microbiology 2019-07, Vol.76 (7), p.835-841
• Main Authors: Lim, Yun Kyong, Park, Soon-Nang, Shin, Jeong Hwan, Chang, Young-Hyo, Shin, Yeseul, Paek, Jayoung, Kim, Hongik, Kook, Joong-Ki
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biotechnology; Dental plaque; DNA-directed RNA polymerase; Fatty acids; Genomes; Gum disease; Lesions; Life Sciences; Microbiology; New species; Nucleotide sequence; Periodontitis; Phylogenetics; Phylogeny; Ribonucleic acid; RNA; RNA polymerase; RpoB protein; rRNA 16S; Sebastes constellatus; Streptococcus; Streptococcus anginosus; Streptococcus infections; Superoxide dismutase
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-019-01695-8

A novel facultative anaerobic and Gram-stain-positive coccus, designated strain ChDC F135 T , was isolated from human subgingival dental plaque of periodontitis lesion and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene (16S rDNA) sequence of strain ChDC F135 T was closest to that of Streptococcus sinensis HKU4 T (98.2%), followed by Streptococcus intermedia SK54 T (97.0%), Streptococcus constellatus NCTC11325 T (96.0%), and Streptococcus anginosus NCTC 10713 T (95.7%). In contrast, phylogenetic analysis based on the superoxide dismutase gene ( sodA ) and the RNA polymerase beta-subunit gene ( rpoB ) showed that the nucleotide sequence similarities of strain ChDC F135 T were highly similar to the corresponding genes of S. anginosus NCTC 10713 T (99.2% and 97.6%, respectively), S. constellatus NCTC11325 T (87.8% and 91.4%, respectively), and S. intermedia SK54 T (85.8% and 91.2%, respectively) rather than those of S. sinensis HKU4 T (80.5% and 82.6%). The complete genome of strain ChDC F135 T consisted of 1,901,251 bp and the G+C content was 38.9 mol %. Average nucleotide identity value between strain ChDC F135 T and S. sinensis HKU4 T or S. anginosus NCTC 10713 T were 75.7% and 95.6%, respectively. The C 14:0 composition of the cellular fatty acids of strain ChDC F135 T (32.8%) was different from that of S. intermedia (6–8%), S. constellatus (6–13%), and S. anginosus (13–20%). Based on the results of phylogenetic and phenotypic analysis, strain ChDC F135 T (= KCOM 2412 T  = JCM 33300 T ) was classified as a type strain of a novel species of the genus Streptococcus , for which we proposed the name Streptococcus periodonticum sp. nov.