Mycobacterium tuberculosis Rv0191 is an efflux pump of major facilitator superfamily transporter regulated by Rv1353c

• Published in: Archives of biochemistry and biophysics 2019-05, Vol.667, p.59-66
• Main Authors: Li, Xue, Li, Ping, Ruan, Cao, Xie, Long xiang, Gu, Yinzhong, Li, Jiang, Yi, Qin, Lv, Xi, Xie, Jianping
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Chloramphenicol; Chloramphenicol - pharmacokinetics; Chloramphenicol - pharmacology; Drug Resistance, Bacterial - genetics; Efflux pump; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genes, Bacterial; Humans; Major facilitator superfamily; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; Mycobacterium smegmatis - drug effects; Mycobacterium smegmatis - genetics; Mycobacterium smegmatis - metabolism; Mycobacterium tuberculosis; Mycobacterium tuberculosis - drug effects; Mycobacterium tuberculosis - genetics; Mycobacterium tuberculosis - metabolism; Promoter Regions, Genetic; Rv0191
• ISSN: 0003-9861; 1096-0384
• DOI: 10.1016/j.abb.2019.04.010

Development of extensively drug resistant (XDR) strains and multidrug resistant (MDR) in Mycobacterium tuberculosis is caused by an efflux mechanism of antibiotics in the bacteria. Rv0191, predicted to a major facilitator superfamily transporter of efflux pump, contributes to elevated expression in some clinical isolates. To characterize the role of Rv0191 which might be involved in antibiotics resistance, Mycobacterium smegmatis was taken as a type strains to do drug susceptibility, ethidium bromide (EB) accumulation assay and electrophoretic mobility shift assay. M. smegmatis Ms0232 mutant became more susceptible to chloramphenicol and showed different cell surface properties. Rv1353c, a TetR family transcription factor, can downregulate the transcription of Rv0191. Rv1353c overexpression strain became more sensitive to chloramphenicol. Together, these findings indicate that Rv1353c encodes a transcriptional repressor that directly interacts with the Rv0191 promoter and modulates the expression of Rv0191. This provided a new player in mycobacteria chloramphenicol resistance. •M. tuberculosis Rv0191 can increase the chloramphenicol resistance.•Rv0191 can serve as a functional MFS transporter which is the major contributor to chloramphenicol resistance.•Chloramphenicol upregulate the transcription of Ms0232.•Rv1353c negatively regulates the transcription of Ms0232 upon chloramphenicol exposure.