Methadone enhances the effectiveness of 5‐aminolevulinic acid‐based photodynamic therapy for squamous cell carcinoma and glioblastoma in vitro

Although having shown promising clinical outcomes, the effectiveness of 5‐aminolevulinic acid‐based photodynamic therapy (ALA‐PDT) for squamous cell carcinoma (SCC) and glioblastoma remains to be improved. The analgesic drug methadone is able to sensitize various tumors to chemotherapy. In this in v...

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Bibliographic Details
Published in:Journal of biophotonics 2019-10, Vol.12 (10), p.e201800468-n/a
Main Authors: Shi, Lei, Buchner, Alexander, Pohla, Heike, Pongratz, Thomas, Rühm, Adrian, Zimmermann, Wolfgang, Gederaas, Odrun A., Zhang, Linglin, Wang, Xiuli, Stepp, Herbert, Sroka, Ronald
Format: Article
Language:English
Subjects:
5‐aminolevulinic acid‐based photodynamic therapy (ALA‐PDT)
Aminolevulinic acid
Analgesics
Apoptosis
Biotechnology
Brain cancer
Cell cycle
cell cycle phase
Cell survival
Chemotherapy
Cytotoxicity
Fluorescence
G1 phase
Glioblastoma
Methadone
Photodynamic therapy
Protoporphyrin
Protoporphyrin IX
Squamous cell carcinoma
squamous cell carcinoma (SCC)
Survival
Tumors
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Summary:Although having shown promising clinical outcomes, the effectiveness of 5‐aminolevulinic acid‐based photodynamic therapy (ALA‐PDT) for squamous cell carcinoma (SCC) and glioblastoma remains to be improved. The analgesic drug methadone is able to sensitize various tumors to chemotherapy. In this in vitro study, the influence of methadone to the effectiveness of ALA‐PDT for SCC (FADU) and glioblastoma (A172) was investigated on the protoporphyrin IX (PpIX) fluorescence, survival rates, apoptosis, and cell cycle phase, each with or without the presence of methadone. The production of PpIX was increased by methadone in FADU cells while it was decreased in A172 cells. The survival rates of both cell lines treated by ALA‐PDT were significantly reduced by the combination with methadone (P 
ISSN:1864-063X
1864-0648
DOI:10.1002/jbio.201800468