Regulating effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis

• Published in: International immunopharmacology 2019-08, Vol.73, p.193-200
• Main Authors: Shen, Jian, Cheng, Jiazheng, Zhu, Shengguo, Zhao, Jun, Ye, Qingyan, Xu, Yinyin, Dong, Huilin, Zheng, Xianhui
• Format: Article
• Language: English
• Subjects: Animal tissues; Animals; Apoptosis; Apoptosis Regulatory Proteins - metabolism; Baicalin; Bax protein; Bcl-2 protein; Colitis, Ulcerative - metabolism; Colitis, Ulcerative - pathology; Colon; Colon - drug effects; Colon - metabolism; Colon - pathology; Flavonoids - pharmacology; I-kappa B Kinase - metabolism; IKK protein; IKK/IKB/NF-kB signaling pathway; IL-1β; Inflammatory bowel disease; Interleukin-1beta - metabolism; Male; Mice; NF-kappa B - metabolism; NF-κB protein; Oxidative Stress - drug effects; Peroxidase - metabolism; Proteins; Rats, Sprague-Dawley; RAW 264.7 Cells; Signal transduction; Signal Transduction - drug effects; Signaling; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-α; Ulcerative colitis; Western blotting
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2019.04.052

This study was aimed to explore effect of baicalin on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in rats with ulcerative colitis (UC). Histopathological observation and scores of colon tissue were performed in the UC rat model. IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins were measured by Western blotting. Baicalin significantly increased the activity of SOD, CAT and GSH-Px in colon tissue of rats with UC, but significantly decreased the content of MDA, IL-1β, MPO, PEG2 and TNF-α in colon tissue of rats with UC. In the molecular mechanism, baicalin significantly decreased the expression of cleaved-caspase3, cleaved-caspase9, Bcl-2/Bax, cyt-c, NF-kB p-65, p-IKKβ/IKKβ and p-IKBα/IKBα. Baicalin could significantly inhibit p-IKBα/IKBα content change, but had no significant effect on p-IKKβ/IKKβ. Baicalin may have a regulating effect on IKK/IKB/NF-kB signaling pathway and apoptosis-related proteins in UC rats. •Baicalin had a protective effect on oxidative stress injury in rats of UC model.•Baicalin had anti-inflammatory effects.•Baicalin had significant effect on colonic cell apoptosis induced by UC.•Baicalin had a significant effect on colonic tissue inflammation in rats of UC model.•Baicalin may be through inhibition of changes in p-IKBα/IKBα content.