Occurrence of putative endornaviruses in non-cultivated plant species in South Louisiana

Extraction and electrophoretic analysis of viral dsRNA from plants has been used successfully to detect infections by RNA viruses. We used this approach as an initial tool to test non-cultivated plant species for the presence of endornaviruses. Foliar samples were collected from symptomless plants i...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2019-07, Vol.164 (7), p.1863-1868
Main Authors: Herschlag, Rachel, Escalante, Cesar, de Souto, Eliezer Rodrigues, Khankhum, Surasak, Okada, Ryo, Valverde, Rodrigo A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Double-stranded RNA
Endornaviridae
Flowers & plants
Geographical variations
Indigenous species
Infectious Diseases
Introduced species
Medical Microbiology
Plant extracts
Polymerase chain reaction
RNA viruses
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Extraction and electrophoretic analysis of viral dsRNA from plants has been used successfully to detect infections by RNA viruses. We used this approach as an initial tool to test non-cultivated plant species for the presence of endornaviruses. Foliar samples were collected from symptomless plants in various locations within East Baton Rouge Parish, Louisiana, USA, and tested for viral dsRNA. After testing 208 plant species belonging to 74 families, five ( Geranium carolinianum , Hydrocotyle umbellata , H. prolifera, Sorghum halepense , and Sisyrinchium atlanticum ) yielded dsRNAs similar in size to the dsRNAs of members of the family Endornaviridae . The endornavirus nature of the dsRNAs was confirmed by reverse-transcription PCR (RT-PCR) and sequencing the RT-PCR products. Sequence data were used to determine relationships of the putative endornaviruses to members of the family Endornaviridae . The putative endornaviruses were detected in both native and introduced plants species. This is the first survey on the occurrence of endornaviruses in non-cultivated plant species.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-019-04270-5