Insights into thermodynamic mechanisms driving bisphenol A (BPA) binding to extracellular polymeric substances (EPS) of activated sludge

• Published in: The Science of the total environment 2019-08, Vol.677, p.502-510
• Main Authors: Yan, Zi-run, Zhu, Yu-ying, Meng, Hui-shan, Wang, Si-yuan, Gan, Li-hong, Li, Xiu-yan, Xu, Juan, Zhang, Wei
• Format: Article
• Language: English
• Subjects: Activated sludge; Bisphenol A (BPA); Extracellular polymeric substances (EPS); Fluorescence quenching; Isothermal titration calorimetry
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2019.04.413

Bisphenol A (BPA) in wastewater has high risks of causing biological feminization. During the wastewater treatment process, large amounts of BPA are accumulated in activated sludge. However, the mechanisms of BPA interacted with activated sludge are still unclear. Especially, the roles of extracellular polymeric substances (EPS), which are major components of activated sludge, in the removal of BPA have never been concerned. In this study, the binding interactions between sludge EPS and BPA are explored combining fluorescence spectroscopy and dynamic light scattering. The thermodynamic mechanisms driving the binding behavior of BPA to EPS are illustrated by isothermal titration calorimetry. The results indicate that the binding interaction between BPA and EPS is spontaneous. BPA mainly binds with the proteins of EPS by hydrophobic association. The random-coiled structure of EPS transforms into relatively condensed cores after binding with BPA. A neutral pH, high ionic strength, and high temperature promote the binding process, facilitating to stabilize BPA in sludge EPS. This study provides new insights into the roles of sludge EPS in the migration and removal of BPA in activated sludge system. [Display omitted] •Bisphenol A mainly bind with the proteins components of EPS.•Random-coiled EPS transforms into condensed cores after binding with bisphenol A.•Binding of bisphenol A to EPS is primarily driven by hydrophobic association.•Neutral pH, high ionic strength and high temperature promote the binding process.