Synthesis of magnetite hybrid nanocomplexes to eliminate bacteria and enhance biofilm disruption

• Published in: Biomaterials science 2019-06, Vol.7 (7), p.2833-284
• Main Authors: Zhang, Chao, Du, Caijuan, Liao, Jian-You, Gu, Yunhao, Gong, Yuzhu, Pei, Jie, Gu, Hongwei, Yin, Dong, Gao, Lizeng, Pan, Yue
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Bacteria; Biocompatibility; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Cell Survival - drug effects; Chemistry Techniques, Synthetic; Disruption; Dose-Response Relationship, Drug; Drug resistance; E coli; Escherichia coli - drug effects; Escherichia coli - physiology; Ferric Compounds - chemistry; Ferrosoferric Oxide - chemical synthesis; Ferrosoferric Oxide - chemistry; Ferrosoferric Oxide - pharmacology; Image enhancement; Iron oxides; Magnetic fields; Magnetic properties; Magnetite; Materials Testing; Metal Nanoparticles - chemistry; Mice; Nanocomposites; Nanoparticles; Nanotechnology; NIH 3T3 Cells; Oxides; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - physiology; Seeds; Silver; Silver - chemistry; Synthesis
• ISSN: 2047-4830; 2047-4849
• DOI: 10.1039/c9bm00057g

Bacteria can increase drug resistance by forming bacterial biofilms. Once the biofilm is formed, it becomes difficult to remove or kill the related bacteria completely by antibiotics and other antibacterial agents because these antibacterial agents cannot easily break through the biofilm matrix barrier and reach the internal bacteria. Therefore, we synthesized magnetite hybrid nanocomplexes that can penetrate and disrupt bacterial biofilms. The obtained nanocomposites are composed of multinucleated iron oxides and Ag seeds. The outer iron oxides can help the internal Ag nanoparticles penetrate the bacterial biofilms, hence killing the internal bacteria and disrupting the biofilms. We took advantage of E. coli and P. aeruginosa bacteria to test the antibacterial properties of the magnetite hybrid nanocomplexes. When planktonic E. coli and P. aeruginosa bacteria were incubated with 100 μg mL −1 magnetite hybrid nanocomplexes for 30 min, almost all the bacteria were killed. When the obtained biofilms of E. coli and P. aeruginosa were treated with magnetite hybrid nanocomplexes (10 μg mL −1 and 100 μg mL −1 ), the survival of E. coli and P. aeruginosa biofilms with a magnetic field showed a big decrease compared with that without a magnetic field. Therefore, the as-synthesized nanocomposites have promising potential as antimicrobial agents for killing bacteria and disrupting biofilms in the presence of a magnetic field, and thus should be further studied for a wide range of antibacterial applications. Magnetite hybrid nanocomplexes are fabricated to eliminate bacteria and enhance biofilm disruption in the presence of a magnetic field.