Comparison of five selective enrichment broths and two selective agars for recovery of Vibrio vulnificus from oysters

• Published in: Journal of food protection 1992-05, Vol.55 (5), p.356-359
• Main Authors: SLOAN, E. M, HAGEN, C. J, LANCETTE, G. A, PEELER, J. T, SOFOS, J. N
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Fish and seafood industries; Food industries; Fundamental and applied biological sciences. Psychology; Mollusca; Vibrio vulnificus
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028X-55.5.356

The Bacteriological Analytical Manual (6th edition) specifies use of glucose-salt-teepol (GST) broth for detection of Vibrio vulnificus and other halophilic vibrios in seafood. Since teepol is no longer commercially available, this study compared five enrichment broths for their ability to recover V. vulnificus . Ten samples of seeded oysters were analyzed using a three-tube MPN and enriched in each of five broths in duplicate. Broth cultures were then streaked onto cellobiose-polymyxin B-colistin (CPC) agar and sodium dodecyl sulfate-polymyxin B-sucrose (SDS) agar plates. Average (± standard error) recovery (log MPN/g) from each broth was as follows: Alkaline-peptone-water (APW), 4.16 ± 0.20; Marine (MRN) broth, 3.63 ± 0.16; Horie's broth, 2.88 ± 0.17; Monsur's broth, 2.43 ± 0.16; and GST broth, 1.28 ± 0.28. APW and MRN broths yielded significantly (P