Utilization of agro-industrial waste for production of Transglutaminase from Streptomyces mobaraensis

• Published in: Bioresource technology 2019-09, Vol.287, p.121391-121391, Article 121391
• Main Authors: Fatima, Syeda Warisul, Tiwari, Rameshwar, Khare, Sunil K.
• Format: Article
• Language: English
• Subjects: Casein cross-linking; Microbial Transglutaminase; Response surface methodology; Solid state fermentation; Streptomyces mobaraensis
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2019.121391

[Display omitted] •Bioprocess development of microbial TGase production using agro-industrial wastes.•Agro-waste: wheat bran used as an inexpensive substrate for TGase production by SSF.•Critical factors for TGase turnout were Protease, MgCl2, CTAB and Inoculation size.•Protease activation coupled with CTAB led to effective secretion of TGase.•Sequential statistical optimization through RSM increased 4-fold TGase production. This work studied the production of Transglutaminase (TGase) using wheat bran as carbon source. The medium components and culture conditions were optimized by statistical Box-Behnken response surface methodology. The release of active Transglutaminase was enhanced by adding (i) protease to remove pro-region to make inactive enzyme to active form, (ii) Cetyl trimethyl ammonium bromide (CTAB) which facilitated more secretion. Under finally optimized conditions viz. 5 g wheat bran, protease: 39.14 U, magnesium chloride (MgCl2): 0.10 M, CTAB: 0.08% and inoculation size: 2% led to 4-fold (12.949 ± 0.061 IU/g) increased TGase production over that of un-optimized conditions. The application of TGase was shown to be useful in effective casein cross-linking.