V617F‐independent upregulation of JAK2 gene expression in patients with inflammatory bowel disease

Background Inflammatory bowel disease (IBD) is one of the most important immune‐mediated disorders of the gastrointestinal tract. Besides, IBD is associated with numerous extraintestinal complications such as venous thromboembolism (VTE), an important risk factor for vascular complications, which re...

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Bibliographic Details
Published in:Journal of cellular biochemistry 2019-09, Vol.120 (9), p.15746-15755
Main Authors: Asadzadeh‐Aghdaei, Hamid, Mashayekhi, Kazem, Koushki, Khadijeh, Azimzadeh, Pedram, Rostami‐Nejad, Mohammad, Amani, Davar, Chaleshi, Vahid, Haftcheshmeh, Saeed Mohammadian, Sahebkar, Amirhossein, Zali, Mohammad Reza
Format: Article
Language:English
Subjects:
Complications
Deoxyribonucleic acid
DNA
Gastrointestinal system
Gastrointestinal tract
Gene expression
Inflammatory bowel disease
Inflammatory bowel diseases
inflammatory bowel diseases (IBD)
Intestine
Janus kinase
Janus kinase 2
Janus kinase 2 (JAK2)
Kinases
Leukocytes (mononuclear)
Morbidity
Mutation
Pathogenesis
Peripheral blood mononuclear cells
Point mutation
Polymerase chain reaction
Polymorphism
Restriction fragment length polymorphism
Risk analysis
Risk factors
Thromboembolism
Thrombosis
Transcription
Up-regulation
V617F
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Summary:Background Inflammatory bowel disease (IBD) is one of the most important immune‐mediated disorders of the gastrointestinal tract. Besides, IBD is associated with numerous extraintestinal complications such as venous thromboembolism (VTE), an important risk factor for vascular complications, which results in the increased morbidity and mortality. The JAK2 (Janus kinase 2) V617F mutation is a well‐known point mutation which is involved in the pathogenesis of IBD, and VTE. Therefore, the aims of this study were to evaluate expression of JAK2 and association of V617F mutation in JAK2 of Iranian patients with IBD. Methods Two hundred and forty‐six patients with IBD (209 UC and 37 CD) and 206 healthy controls were enrolled in this study. The genomic DNA and total RNA were extracted from peripheral blood mononuclear cells (PBMCs). Then, the JAK2 V617F mutation detection was performed using the restriction fragment length polymorphism (RFLP) method. In addition, the JAK2 mRNA expression was evaluated using a quantitative polymerase chain reaction (q‐PCR) using the SYBR Green assay. Results There was no association of V61F mutation in patients with IBD with or without thrombosis compared with healthy control. However, the relative mRNA expression of JAK2 was significantly upregulated in patients with IBD in comparison with healthy control (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28844