Molecular investigation of tick-borne infections in cattle from Xinjiang Uygur Autonomous Region, China

• Published in: Parasitology international 2020-02, Vol.74, p.101925-101925, Article 101925
• Main Authors: Li, Yongchang, Li, Jixu, Chahan, Bayin, Guo, Qingyong, Zhang, Yang, Moumouni, Paul Franck Adjou, Lee, Seung-Hun, Liu, Mingming, Galon, Eloiza May, Guo, Huanping, Gao, Yang, Song, Ruiqi, Li, Min, Tumwebaze, Maria Agnes, Benedicto, Byamukama, Xuan, Xuenan
• Format: Article
• Language: English
• Subjects: Anaplasma - genetics; Anaplasma - isolation & purification; Anaplasmosis - epidemiology; Animals; Babesia - genetics; Babesia - isolation & purification; Babesiosis - epidemiology; Cattle; Cattle Diseases - epidemiology; Cattle Diseases - microbiology; Cattle Diseases - parasitology; China; China - epidemiology; Coxiella burnetii - genetics; Coxiella burnetii - isolation & purification; DNA, Bacterial - genetics; DNA, Protozoan - genetics; PCR; Phylogeny; Q Fever - epidemiology; Q Fever - veterinary; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Tick-borne diseases; Tick-Borne Diseases - epidemiology; Tick-Borne Diseases - veterinary; Ticks - microbiology; Xinjiang Uygur Autonomous Region (XUAR)
• ISSN: 1383-5769; 1873-0329
• DOI: 10.1016/j.parint.2019.05.003

Tick-borne diseases cause significant losses to livestock production in tropical and subtropical regions. However, information about the tick-borne infections in cattle in Xinjiang Uygur Autonomous Region (XUAR), northwestern China, is scarce. In this study, nested polymerase chain reaction (PCR) assays and gene sequencing were used to detect and analyze epidemiological features of Babesia bovis, B. bigemina, Coxiella burnetii and Anaplasma bovis infections in XUAR. Out of 195 samples tested, 24 (12.3%), 67 (34.4%), 40 (20.5%) and 10 (5.1%) were positive for B. bovis, B. bigemina, C. burnetii and A. bovis, respectively. Sequencing analysis indicated that B. bovis SBP-4, B. bigemina Rap1a, C. burnetii htpB and A. bovis 16S rRNA genes from XUAR showed 99%–100% identity with documented isolates from other countries. Phylogenetic analyses revealed that B. bovis SBP-4, B. bigemina Rap1a, C. burnetii htpB and A. bovis 16S rRNA gene sequences clustered in the same clade with isolates from other countries. To the best of our knowledge, this is the first report of C. burnetii infection of cattle in XUAR. Furthermore, this study provides important data for understanding the distribution of tick-borne pathogens, and is expected to improve the approach for prevention and control of tick-borne diseases in China. [Display omitted] •Babesia bovis, B. bigemina, Coxiella burnetii and Anaplasma bovis were detected in cattle in XUAR•72.3% samples were found to be infected with one to three pathogens•This is the first report of C. burnetii infection of cattle in XUAR•This study improve the approach for prevention and control of tick-borne diseases in China