Precursor-feeding and altered-growth conditions reveal novel blue pigment production by Rubrivivax benzoatilyticus JA2

Objective To explore the secondary metabolite biosynthetic potential of Rubrivivax benzoatilyticus JA2 using a new metabolite mining strategy. Results Combination of precursor-feeding and altered growth conditions were used to mine new biomolecules. Strain JA2 utilised l -phenylalanine as sole sourc...

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Bibliographic Details
Published in:Biotechnology letters 2019-07, Vol.41 (6-7), p.813-822
Main Authors: Mekala, Lakshmi Prasuna, Mohammed, Mujahid, Chintalapati, Sasikala, Chintalapati, Venkata Ramana
Format: Article
Language:English
Subjects:
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biomolecules
Biotechnology
Feeding
Growth conditions
Life Sciences
Metabolites
Microbiology
Microorganisms
Mining
Original Research Paper
pH effects
Phenylalanine
Pigments
Precursors
Stable isotopes
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Summary:Objective To explore the secondary metabolite biosynthetic potential of Rubrivivax benzoatilyticus JA2 using a new metabolite mining strategy. Results Combination of precursor-feeding and altered growth conditions were used to mine new biomolecules. Strain JA2 utilised l -phenylalanine as sole source of nitrogen and showed pigments production only under phenylalanine-amended aerobic cultures. Stable isotope based precursor feeding studies indicated the blue pigment consists of 4-phenyl rings derived from l -phenylalanine. The purified blue pigment displayed characteristic visible-absorption and pH-dependent color variations. Precursor-feeding under altered growth conditions activated the plausible novel aromatic pigment production in strain JA2. Conclusion Our approach unraveled the previously unknown pigment synthesis in strain JA2 and demonstrated the potential of mining strategy in discovering the hidden secondary metabolite repertoire in microorganisms.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-019-02682-6