Fast, Efficient, and Targeted Liposome Delivery Mediated by DNA Hybridization

Safety and efficacy, two significant parameters in drug administration, can be improved by site‐specific delivery approaches. Here a fast, efficient, and targeted liposome delivery system steered by a DNA hybridization recognition mechanism is presented. For this purpose, lipid‐terminated DNA is ins...

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Bibliographic Details
Published in:Advanced healthcare materials 2019-07, Vol.8 (14), p.e1900389-n/a
Main Authors: Li, Hongyan, Liu, Qing, Crielaard, Bart J., de Vries, Jan W., Loznik, Mark, Meng, Zhuojun, Yang, Xintong, Göstl, Robert, Herrmann, Andreas
Format: Article
Language:English
Subjects:
Caveolae
Cell membranes
Complementary DNA
Deoxyribonucleic acid
DNA
DNA hybridization
Drug delivery
Drug delivery systems
Endocytosis
Gene sequencing
Hybridization
Lipids
Liposomes
Low temperature
Membranes
Molecular biology
Nucleotide sequence
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Summary:Safety and efficacy, two significant parameters in drug administration, can be improved by site‐specific delivery approaches. Here a fast, efficient, and targeted liposome delivery system steered by a DNA hybridization recognition mechanism is presented. For this purpose, lipid‐terminated DNA is inserted in both liposome and cell membranes by simple mixing of the components. Cellular accumulation of cargo encapsulated in the liposomal core is substantially enhanced when the DNA sequence on the cell is complementary to that on the liposome. Additionally, in mixed cell populations, liposomes discriminate targets by their complementary DNA sequences. Exposure of cells to low temperature and endocytosis inhibitors suggests a caveolae‐dependent endocytosis uptake pathway. Mechanistically, hybridization between DNA strands spatially traps liposomes and cell membranes in close proximity, consequently increases the local liposome concentration, and thereby enhances cellular uptake of liposomes and their payload. This programmable delivery system might contribute to new applications in molecular biology and drug delivery. Hybridization of complementary amphiphilic DNA anchored to liposomal and cellular membranes enables the targeted delivery of cargo‐loaded liposomes to cells. The liposomes enter cells by caveolae‐mediated endocytosis and release their cargo into lysosome and cytosol. This DNA hybridization‐based delivery is not limited to liposomes, can prospectively be applied to other nanostructures, and find application for the treatment of lysosome‐related diseases.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201900389