Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139

A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection o...

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Bibliographic Details
Published in:Molecular and cellular probes 2019-06, Vol.45, p.65-67
Main Authors: Izumiya, Hidemasa, Morita, Masatomo, Arakawa, Eiji, Ngo, Tuan Cuong, Nguyen, Hoai Thu, Nguyen, Dong Tu, Ohnishi, Makoto
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Cholera
Cholera - diagnosis
Environmental Monitoring
Humans
LAMP
Limit of Detection
Molecular Diagnostic Techniques - methods
Nucleic Acid Amplification Techniques - methods
O1/O139
Vibrio cholerae O1 - genetics
Vibrio cholerae O1 - isolation & purification
Vibrio cholerae O139 - genetics
Vibrio cholerae O139 - isolation & purification
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Summary:A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium. •Multiple LAMP-based methods for rapid detection of cholera-causing agents were developed.•Combination of these LAMP methods can be applied in molecular diagnosis or detection of V. cholerae O1 or O139.
ISSN:0890-8508
1096-1194
DOI:10.1016/j.mcp.2019.05.001