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Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139
A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection o...
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| Published in: | Molecular and cellular probes 2019-06, Vol.45, p.65-67 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c353t-6153a1bde267e6262d3176c7a3fe87b1376d611244b15937a4890eb581902f533 |
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| cites | cdi_FETCH-LOGICAL-c353t-6153a1bde267e6262d3176c7a3fe87b1376d611244b15937a4890eb581902f533 |
| container_end_page | 67 |
| container_issue | |
| container_start_page | 65 |
| container_title | Molecular and cellular probes |
| container_volume | 45 |
| creator | Izumiya, Hidemasa Morita, Masatomo Arakawa, Eiji Ngo, Tuan Cuong Nguyen, Hoai Thu Nguyen, Dong Tu Ohnishi, Makoto |
| description | A loop-mediated isothermal amplification assay was developed. It was designed for recognizing Vibrio cholerae O1/O139, where atpA, rfbN, and wfbR genes were adopted. The assay specifically detected the target with sensitivities of 5–67 copies per reaction in 1 h. The assay will aid rapid detection of the cholera bacterium.
•Multiple LAMP-based methods for rapid detection of cholera-causing agents were developed.•Combination of these LAMP methods can be applied in molecular diagnosis or detection of V. cholerae O1 or O139. |
| doi_str_mv | 10.1016/j.mcp.2019.05.001 |
| format | article |
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| fulltext | fulltext |
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| ispartof | Molecular and cellular probes, 2019-06, Vol.45, p.65-67 |
| issn | 0890-8508 1096-1194 |
| language | eng |
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| source | ScienceDirect Freedom Collection |
| subjects | Bacterial Proteins - genetics Cholera Cholera - diagnosis Environmental Monitoring Humans LAMP Limit of Detection Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods O1/O139 Vibrio cholerae O1 - genetics Vibrio cholerae O1 - isolation & purification Vibrio cholerae O139 - genetics Vibrio cholerae O139 - isolation & purification |
| title | Development of a loop-mediated isothermal amplification assay for Vibrio cholerae O1 and O139 |
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