Valuable method for production of oral vaccine by using alginate and chitosan against Lactococcus garvieae/Streptococcus iniae in rainbow trout (Oncorhynchus mykiss)

• Published in: Fish & shellfish immunology 2019-07, Vol.90, p.431-439
• Main Authors: Halimi, Mostafa, Alishahi, Mojtaba, Abbaspour, Mohammad Reza, Ghorbanpoor, Masoud, Tabandeh, Mohammad Reza
• Format: Article
• Language: English
• Subjects: Chitosan-alginate; Ionotropic gelation; Lactococcus garvieae; Oral vaccination; Rainbow trout; Streptococcus iniae
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2019.05.020

The effectiveness of ionotropic gelation method (by combining alginate and chitosan) vaccine against Lactococcus garvieae and Streptococcus iniae was examined in rainbow trout. Fish were separated into four groups and fed the distinctive examined feeds. Our groups were included: A) fish immunized by chitosan-alginate coated vaccine, B) fish immunized by non-coated vaccine, C) fish feed by chitosan-alginate coated pellets without vaccine and D) fish feed by basic diet (non-coated and without vaccine). In groups A and B, the vaccination was carried out for 14 days. Fish of group C, like groups A and B were fed 14 days with pellets covered with chitosan-alginate without vaccine and a short time later they were fed with control diet. On day 0, 20, 40 and 60 of the trial, serum samples were extracted. Fish were challenged with L. garvieae and S. iniae after 60 days of research. Innate immunity components containing complement activity, total protein and IgM appeared no significant changes nearly in all groups during the 60 days that the examination finished. Although, bactericidal activity and lysozyme activity demonstrated a significant increase on days 20, 40 and 60 in group A compared to control groups (C and D) (P