Comparison of three selective enrichment methods for the isolation of Listeria monocytogenes from naturally contaminated foods

Three selective enrichment procedures--the U.S. Food and Drug Administration (FDA) method, the U.S. Department of Agriculture (USDA) method, and the Netherlands Government Food Inspection Service (NGFIS) method--were compared for isolating Listeria monocytogenes from contaminated foods. The foods we...

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Bibliographic Details
Published in:Journal of food protection 1992-12, Vol.55 (12), p.952-959
Main Authors: Hayes, P.S. (Meningitis and Special Pathogens Branch, National Center for Infectious Diseases, Atlanta, GA), Graves, L.M, Swaminathan, B, Ajello, G.W, Malcolm, G.B, Weaver, R.E, Ransom, R, Deaver, K, Plikaytis, B.D
Format: Article
Language:English
Subjects:
ALIMENTOS
ANALISIS BIOLOGICO
ANALYSE BIOLOGIQUE
Biological and medical sciences
CONTAMINACION
CONTAMINATION
EXPERIMENTATION EN LABORATOIRE
EXPERIMENTOS EN LABORATORIO
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
LISTERIA MONOCYTOGENES
METHODE
METODOS
PRODUIT ALIMENTAIRE
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Summary:Three selective enrichment procedures--the U.S. Food and Drug Administration (FDA) method, the U.S. Department of Agriculture (USDA) method, and the Netherlands Government Food Inspection Service (NGFIS) method--were compared for isolating Listeria monocytogenes from contaminated foods. The foods were obtained from the refrigerators of patients with culture-proven listeriosis who were identified through multistate active surveillance in a U.S. population of 19 million. The study was designed to identify foods that may be important in transmission of L. monocytogenes in sporadic cases of human listeriosis. Of 899 foods analyzed by all three methods, 121 were positive for L. monocytogenes by at least one method. The three enrichment methods detected L. monocytogenes in 65% (FDA), 74% (USDA), and 74% (NGFIS) of the foods shown to contain L. monocytogenes. The differences among the three methods were not statistically significant. However, the recovery of L. monocytogenes by a combination of any two methods (USDA-FDA 88%, USDA-NGFIS 91%, FDA-NGFIS 87%) was significantly better than that by one method alone (p 0.02). The differences among the combinations of methods were not statistically significant. These results suggest that at least two enrichment methods must be used in combination to recover L. monocytogenes from contaminated foods with a success rate near 90%. Correlations were observed between negative results and low ( 0.3 CFU/g) level of L. monocytogenes contamination for the USDA (p 0.001) and NGFIS (p 0.001) methods. A similar but somewhat weaker association was observed for the FDA method (p 0.06)
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-55.12.952