Use of reference materials (Spray-dried milk artificially contaminated with Salmonella typhimurium) to validate detection methods for Salmonella

Reference materials consisting of 0.28 g of spray-dried milk artificially contaminated with Salmonella typhimurium and contained in gelatin capsules (mean contamination level 4.2 salmonellae per capsule) were used to validate detection of Salmonella species in food. For this, 10 samples of 75 g of m...

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Bibliographic Details
Published in:Journal of food protection 1992-11, Vol.55 (11), p.855-858
Main Authors: Veld, P.H.I. (National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands), Notermans, S
Format: Article
Language:English
Subjects:
ALIMENTOS
Biological and medical sciences
CONTAMINACION
CONTAMINATION
EXPERIMENTATION EN LABORATOIRE
EXPERIMENTOS EN LABORATORIO
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
METHODE
METODOS
PRODUCTOS LACTEOS
PRODUIT ALIMENTAIRE
PRODUIT LAITIER
SALMONELLA
Salmonella typhimurium
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Description
Summary:Reference materials consisting of 0.28 g of spray-dried milk artificially contaminated with Salmonella typhimurium and contained in gelatin capsules (mean contamination level 4.2 salmonellae per capsule) were used to validate detection of Salmonella species in food. For this, 10 samples of 75 g of mayonnaise, milk powder, minced meat, or poultry meat were spiked with reference materials without repair and with reference materials after repair, respectively. It was found that the physiological condition of Salmonella present in the reference material had no influence on the recovery rate of the organism. The recovery rates of Salmonella were tested by applying various methods [ISO-6579 method, SALMONELLA-TEK (Organon Teknika), the modified semisolid Rappaport Vassiliadis agar (MSRV), and the Salmonella rapid test (Oxoid)]. The recovery rates found (averaged on the types of foods tested) were 67% for the Salmonella rapid test, 83% for the MSRV, 86% for the ISO-6579, and 91% for the SALMONELLA-TEK. The use of reference materials is recommended to validate detection of Salmonella species
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-55.11.855