Detection of Leptospire spp. in animals and in environmental samples from peridomestic areas in NicaraguaDetecção de Leptospira spp. em animais e em amostras ambientais de áreas peridomiciliares na Nicarágua

The objective of this study was to determine the epidemiological characteristics of leptospirosis in pets and in humans in peridomestic settings in Nicaragua between 2014 and 2016. The samples were taken in areas where cases were confirmed in humans using non-probabilistic sampling in 10 of the coun...

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Bibliographic Details
Published in:Revista panamericana de salud pública 2018, Vol.42, p.e26-e26
Main Authors: Chávez, Álvaro, Somarriba, Byron Flores, Soto, Aida, Sheleby-Elías, Jessica, Duttmann, Christiane, Jiménez, Eduardo, Pérez, Eveling, Mora, Brenda, Jirón, William
Format: Article
Language:Spanish
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Summary:The objective of this study was to determine the epidemiological characteristics of leptospirosis in pets and in humans in peridomestic settings in Nicaragua between 2014 and 2016. The samples were taken in areas where cases were confirmed in humans using non-probabilistic sampling in 10 of the country's 17 departments. This included 112 urine samples from pets, 129 water samples, and 69 soil samples in order to isolate leptospires in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium. Furthermore, the microscopic agglutination test (MAT) was applied to 263 samples of animal serum, and 88 isolates were analyzed using PCR. In 32.6% (101/310) of the samples, spirochetes were isolated: 23.2% (26/112) in the pet urine, 47.3% (61/129) in water samples, and 20.3% (14/69) in soil samples. Isolation analysis showed significant differences (p
ISSN:1680-5348
DOI:10.26633/RPSP.2018.26