Polyelectrolyte nanocomplexes based on chitosan derivatives for wound healing application

[Display omitted] Wound healing, when compromised, may be guided by biological cues such as Arg-Gly-Asp (RGD), a peptide known to induce cell adhesion and migration, eventually combined with adapted nanocarriers. Three different formulations were prepared and investigated in vitro for topical applic...

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Bibliographic Details
Published in:European journal of pharmaceutics and biopharmaceutics 2019-07, Vol.140, p.100-108
Main Authors: Patrulea, Viorica, Laurent-Applegate, Lee Ann, Ostafe, Vasile, Borchard, Gerrit, Jordan, Olivier
Format: Article
Language:English
Subjects:
Chitosan derivative
Hydrogel
Polyelectrolyte
RGDC
Wound healing
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Summary:[Display omitted] Wound healing, when compromised, may be guided by biological cues such as Arg-Gly-Asp (RGD), a peptide known to induce cell adhesion and migration, eventually combined with adapted nanocarriers. Three different formulations were prepared and investigated in vitro for topical application. All formulations were based on carboxylated and trimethylated chitosan (CMTMC) displaying RGD. The polyelectrolyte nanocomplexes were prepared by mixing two oppositely charged polymers of CMTMC and chondroitin sulfate at different polymer ratios and subsequently characterized by dynamic light scattering and scanning electron microscopy. Hydrogels and foams with a high concentration of RGD-functionalized chitosan (3%) and hyaluronic acid (1.5%) that formed gel-embedded nanocomplexes were developed. In vitro assays showed absence of toxicity, ability to promote proliferation over 7 days and promotion of migration of human dermal fibroblasts treated with any of our formulations. These formulations were shown to be suitable for easy topical application and have the potential to accelerate wound healing.
ISSN:0939-6411
1873-3441
DOI:10.1016/j.ejpb.2019.05.009